Detailed information of TA system TA224440

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2621257..2622845
• Replicon: chromosome
• Accession: NZ_CP086761
• Organism: Klebsiella pneumoniae strain AZS099

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A485W5A2
• Locus tag: LON84_RS12970
• Protein ID: WP_023342812.1
• Coordinates: 2621257..2622579 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LON84_RS12975
• Protein ID: WP_024622638.1
• Coordinates: 2622579..2622845 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LON84_RS12955 (2616313)	2616313..2618814	+	2502	WP_023342811.1	fimbria/pilus outer membrane usher protein	-
LON84_RS12960 (2618802)	2618802..2619806	+	1005	WP_004224758.1	fimbrial protein	-
LON84_RS12965 (2619948)	2619948..2621240	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LON84_RS12970 (2621257)	2621257..2622579	-	1323	WP_023342812.1	type II toxin-antitoxin system HipA family toxin	Toxin
LON84_RS12975 (2622579)	2622579..2622845	-	267	WP_024622638.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LON84_RS12980 (2623110)	2623110..2623436	-	327	WP_004184023.1	hydroxyisourate hydrolase	-
LON84_RS12985 (2623433)	2623433..2623933	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LON84_RS12990 (2623911)	2623911..2625290	-	1380	WP_004184021.1	nucleobase:cation symporter-2 family protein	-
LON84_RS12995 (2625636)	2625636..2626790	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LON84_RS13000 (2626771)	2626771..2627694	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (17-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5358

>T224440 WP_023342812.1 NZ_CP086761:c2622579-2621257 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYEEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEVNAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T224440 NZ_CP120328:c123316-122900 [Salmonella enterica subsp. enterica serovar Muenchen]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9924.30 Da
• Isoelectric Point: 8.5912

>AT224440 WP_024622638.1 NZ_CP086761:c2622845-2622579 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYMKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT224440 NZ_CP120328:c123543-123313 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• AlphaFold DB: A0A485W5A2