Detailed information of TA system TA022444

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1268245..1268716
• Replicon: chromosome
• Accession: NC_009801
• Organism: Escherichia coli E24377A

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: ECE24377A_RS07780
• Protein ID: WP_001303511.1
• Coordinates: 1268245..1268523 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: -
• Locus tag: ECE24377A_RS07785
• Protein ID: WP_012138131.1
• Coordinates: 1268525..1268716 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECE24377A_RS07750	1263530..1265907	-	2378	Protein_1159	3'-5' exoribonuclease	-
ECE24377A_RS07755	1266001..1266192	-	192	WP_001083283.1	lysis protein YdfD	-
ECE24377A_RS07760	1266189..1266377	-	189	WP_000449196.1	cell division inhibition protein DicB	-
ECE24377A_RS07765	1266941..1267150	+	210	WP_001133037.1	hypothetical protein	-
ECE24377A_RS07770	1267151..1267789	-	639	WP_012138129.1	hypothetical protein	-
ECE24377A_RS07775	1267801..1267953	-	153	WP_001394175.1	DUF1391 family protein	-
ECE24377A_RS07780	1268245..1268523	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
ECE24377A_RS07785	1268525..1268716	-	192	WP_012138131.1	hypothetical protein	Antitoxin
ECE24377A_RS29295	1268737..1269108	-	372	WP_001169688.1	hypothetical protein	-
ECE24377A_RS07795	1269207..1269509	+	303	WP_000172738.1	transcriptional regulator	-
ECE24377A_RS07800	1269506..1269931	+	426	WP_000693838.1	Rha family transcriptional regulator	-
ECE24377A_RS07805	1269955..1270902	+	948	WP_000095679.1	helix-turn-helix domain-containing protein	-
ECE24377A_RS07810	1270909..1271655	+	747	WP_000788770.1	ATP-binding protein	-
ECE24377A_RS07815	1271677..1272438	+	762	WP_000450664.1	DUF1627 domain-containing protein	-
ECE24377A_RS07820	1272454..1272876	+	423	WP_001151247.1	DUF977 family protein	-
ECE24377A_RS07825	1272879..1273291	+	413	Protein_1174	DUF4406 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	1256977..1289151	32174

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T22444 WP_001303511.1 NC_009801:c1268523-1268245 [Escherichia coli O139:H28 str. E24377A]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T22444 NC_009801:c1268523-1268245 [Escherichia coli O139:H28 str. E24377A]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7447.47 Da
• Isoelectric Point: 8.6828

>AT22444 WP_012138131.1 NC_009801:c1268716-1268525 [Escherichia coli O139:H28 str. E24377A]
MNRALSPMVSEFETIEQENSYNEWLRAKVAASLADPRPAIPHDEIERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT22444 NC_009801:c1268716-1268525 [Escherichia coli O139:H28 str. E24377A]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAGCGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAATTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE