Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 515237..515855 Replicon chromosome
Accession NC_009801
Organism Escherichia coli E24377A

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag ECE24377A_RS03995 Protein ID WP_001291435.1
Coordinates 515237..515455 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag ECE24377A_RS04000 Protein ID WP_000344800.1
Coordinates 515481..515855 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECE24377A_RS03965 510526..511098 + 573 WP_000779826.1 lipoprotein -
ECE24377A_RS03970 511129..511440 - 312 WP_000409911.1 MGMT family protein -
ECE24377A_RS03975 511819..512172 + 354 WP_000878141.1 DUF1428 family protein -
ECE24377A_RS03980 512214..513764 - 1551 WP_001299455.1 cyclic-guanylate-specific phosphodiesterase PdeB -
ECE24377A_RS03985 513928..514398 - 471 WP_000136192.1 YlaC family protein -
ECE24377A_RS03990 514514..515065 - 552 WP_000102564.1 maltose O-acetyltransferase -
ECE24377A_RS03995 515237..515455 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
ECE24377A_RS04000 515481..515855 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
ECE24377A_RS04005 516401..519550 - 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
ECE24377A_RS04010 519573..520766 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T22443 WP_001291435.1 NC_009801:c515455-515237 [Escherichia coli O139:H28 str. E24377A]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T22443 NC_009801:c515455-515237 [Escherichia coli O139:H28 str. E24377A]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTATACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT22443 WP_000344800.1 NC_009801:c515855-515481 [Escherichia coli O139:H28 str. E24377A]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT22443 NC_009801:c515855-515481 [Escherichia coli O139:H28 str. E24377A]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References