Detailed information of TA system TA224427

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 1432980..1433568
• Replicon: chromosome
• Accession: NZ_CP086759
• Organism: Acinetobacter baumannii strain ACI713

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: A0A3G6Z3M6
• Locus tag: LN920_RS06840
• Protein ID: WP_000438826.1
• Coordinates: 1432980..1433267 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: N9M5I3
• Locus tag: LN920_RS06845
• Protein ID: WP_001983304.1
• Coordinates: 1433254..1433568 (+)
• Length: 105 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN920_RS06815 (LN920_06815)	1428423..1429250	-	828	WP_000073659.1	AraC family transcriptional regulator	-
LN920_RS06820 (LN920_06820)	1429424..1430130	-	707	Protein_1319	IS1 family transposase	-
LN920_RS06825 (LN920_06825)	1430221..1431063	-	843	WP_002002480.1	carbapenem-hydrolyzing class D beta-lactamase OXA-58	-
LN920_RS06830 (LN920_06830)	1431298..1432268	+	971	Protein_1321	IS30 family transposase	-
LN920_RS06835 (LN920_06835)	1432265..1432750	+	486	WP_220385121.1	LysE family transporter	-
LN920_RS06840 (LN920_06840)	1432980..1433267	+	288	WP_000438826.1	BrnT family toxin	Toxin
LN920_RS06845 (LN920_06845)	1433254..1433568	+	315	WP_001983304.1	BrnA antitoxin family protein	Antitoxin
LN920_RS06850 (LN920_06850)	1433696..1434409	+	714	Protein_1325	TonB-dependent receptor ZnuD2	-
LN920_RS06855 (LN920_06855)	1434481..1435571	+	1091	WP_085940413.1	IS4-like element ISAba1 family transposase	-
LN920_RS06860 (LN920_06860)	1435590..1437296	+	1707	Protein_1327	TonB-dependent receptor ZnuD2	-
LN920_RS06865 (LN920_06865)	1437601..1438062	+	462	WP_000761346.1	DIP1984 family protein	-
LN920_RS06870 (LN920_06870)	1438385..1438558	-	174	WP_229997797.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	blaOXA-58	-	1427207..1435571	8364

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11303.72 Da
• Isoelectric Point: 6.0889

>T224427 WP_000438826.1 NZ_CP086759:1432980-1433267 [Acinetobacter baumannii]
MEQYFEWDEAKNRKNQKKHDISFETASLVFEDPLRISIQDRHTNGEERWQTIGRVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERNKYEHG

Nucleotide

• Length: 288 bp

>T224427 NZ_CP120326:50119-50424 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 105 a.a.
• Molecular weight: 12208.84 Da
• Isoelectric Point: 7.2026

>AT224427 WP_001983304.1 NZ_CP086759:1433254-1433568 [Acinetobacter baumannii]
MSMVRYSHKELNEKFGEKQDAEIQRLLAKGTVPDDQLDLSDIPEITDWSNAVRQNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKNHQ

Nucleotide

• Length: 315 bp

>AT224427 NZ_CP120326:49899-50117 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: A0A3G6Z3M6

Antitoxin

• AlphaFold DB: N9M5I3