Detailed information of TA system TA224420

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/-
• Location: 824194..825302
• Replicon: chromosome
• Accession: NZ_CP086728
• Organism: Streptococcus parasuis strain SS20

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: -
• Locus tag: LOD77_RS04280
• Protein ID: WP_274505813.1
• Coordinates: 824433..825302 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LOD77_RS04275
• Protein ID: WP_274505812.1
• Coordinates: 824194..824418 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOD77_RS04255 (LOD77_04245)	820799..821167	+	369	WP_274505809.1	hypothetical protein	-
LOD77_RS04260 (LOD77_04250)	821170..822486	+	1317	WP_274505810.1	hypothetical protein	-
LOD77_RS04265 (LOD77_04255)	822497..823615	+	1119	WP_274505811.1	AAA family ATPase	-
LOD77_RS04270 (LOD77_04260)	823970..824050	+	81	Protein_802	site-specific recombinase	-
LOD77_RS04275 (LOD77_04265)	824194..824418	+	225	WP_274505812.1	helix-turn-helix transcriptional regulator	Antitoxin
LOD77_RS04280 (LOD77_04270)	824433..825302	+	870	WP_274505813.1	nucleotidyltransferase domain-containing protein	Toxin
LOD77_RS04285 (LOD77_04275)	825283..826017	+	735	WP_274505814.1	class I SAM-dependent methyltransferase	-
LOD77_RS04290 (LOD77_04280)	826050..826958	+	909	WP_274505815.1	aminoglycoside 6-adenylyltransferase	-
LOD77_RS04295 (LOD77_04285)	826955..827497	+	543	WP_000627290.1	streptothricin N-acetyltransferase Sat4	-
LOD77_RS04300 (LOD77_04290)	827590..828384	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
LOD77_RS04305 (LOD77_04295)	828660..829232	+	573	WP_001079938.1	HTH domain-containing protein	-
LOD77_RS04310 (LOD77_04300)	829316..829612	+	297	WP_274505816.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	ant(6)-Ia / aph(3')-III	-	816500..842881	26381

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32806.44 Da
• Isoelectric Point: 4.8781

>T224420 WP_274505813.1 NZ_CP086728:824433-825302 [Streptococcus parasuis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQITTELDDENRNNLVVPPGAWGDW
VNGGGWLVINGCHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPKKYAE
RVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T224420 NZ_CP120325:c4165974-4165636 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGAAAATCCTTTCTGCAAATCAGCGGGTGGCGAAGCCATGCCCGCCGCCTGTACTTGTCTGGCAAACGCTACTGACACG
CCTGCTGGAGCAACACTATGGCCTGTCGCTGAACGATACGCCGTTCAGTGATGAAACGGTTATTCAGGAGCATATCAACG
CCGGTATCACGCTGGCGGATGCAGTTAACTTTCTGGTGGAAAAGTACGAACTGGTTCGTATCGATCGCAGAGGGTTTAAC
TGTCAGGAACAGTCGCCTTACCTTCGGGCGGTTGATATTTTACGGGCGCGACAGGCTACTGGTCTGTTGCGGCAAAACCA
GCTTCCTTCGACACGCTGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8351.65 Da
• Isoelectric Point: 4.5357

>AT224420 WP_274505812.1 NZ_CP086728:824194-824418 [Streptococcus parasuis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVIGVKFVQAFILPDGDEIKISNE

Nucleotide

• Length: 225 bp

>AT224420 NZ_CP120325:c4166328-4165999 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGATAAAGAAACCGACACAGTACAGTGGGGACTCAACCGACCTGGTTCTCCTCAATTTAGCGCACGCCTGGTACAAGA
AGGCAAACGCCTACATTATTTAGCTGACCGCGCCGGGATAACAGGCGCTTTCAATGAAGAAGAGTGTCACCAACTGAATG
CCGCATTTCCGCACTTCGTAAAACAAATGGAGCTGATGCTGCTCTCTGGTGAGCTTAACCCCCGCCATGCATATCGCGTA
ACGCTGTACCACAACGAACTGACCTGTGAAGCAGATACGCTCGGTAGCTGTGGCTACGTTTATATAGCGATTTATCCCAC
TCAACGCTAA