Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 3074827..3075964 Replicon chromosome
Accession NZ_CP086726
Organism Enterococcus faecalis strain E533

Toxin (Protein)


Gene name zeta Uniprot ID -
Locus tag LM507_RS15000 Protein ID WP_002401483.1
Coordinates 3075101..3075964 (+) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag LM507_RS14995 Protein ID WP_000301765.1
Coordinates 3074827..3075099 (+) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LM507_RS14960 (3070312) 3070312..3071799 + 1488 WP_010712965.1 type IA DNA topoisomerase -
LM507_RS14965 (3071919) 3071919..3072041 + 123 Protein_2922 peptide-binding protein -
LM507_RS14970 (3072110) 3072110..3072205 + 96 WP_001809736.1 23S rRNA methyltransferase attenuation leader peptide -
LM507_RS14975 (3072330) 3072330..3073067 + 738 WP_002292226.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
LM507_RS14980 (3073237) 3073237..3073497 + 261 Protein_2925 topoisomerase DNA-binding C4 zinc finger domain-containing protein -
LM507_RS14985 (3073600) 3073600..3074496 + 897 WP_002387620.1 ParA family protein -
LM507_RS14990 (3074595) 3074595..3074810 + 216 WP_002295735.1 peptide-binding protein -
LM507_RS14995 (3074827) 3074827..3075099 + 273 WP_000301765.1 antitoxin Antitoxin
LM507_RS15000 (3075101) 3075101..3075964 + 864 WP_002401483.1 zeta toxin family protein Toxin
LM507_RS15005 (3076405) 3076405..3076722 + 318 WP_002333463.1 hypothetical protein -
LM507_RS15010 (3076742) 3076742..3077029 + 288 Protein_2931 DnaJ domain-containing protein -
LM507_RS15015 (3077103) 3077103..3077783 + 681 WP_010710189.1 IS6-like element IS1216 family transposase -
LM507_RS15020 (3077839) 3077839..3078270 + 432 Protein_2933 2-dehydropantoate 2-reductase -
LM507_RS15025 (3078546) 3078546..3079397 + 852 WP_002357480.1 ribosome biogenesis GTPase YlqF -
LM507_RS15030 (3079399) 3079399..3080166 + 768 WP_002357481.1 ribonuclease HII -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn erm(B) / aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III / lsa(E) / lnu(B) - 3047206..3077783 30577


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-213)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32486.06 Da        Isoelectric Point: 6.9965

>T224418 WP_002401483.1 NZ_CP086726:3075101-3075964 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Download         Length: 864 bp

>T224418 NZ_CP120325:3522071-3522289 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT224418 WP_000301765.1 NZ_CP086726:3074827-3075099 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT224418 NZ_CP120325:3521669-3522043 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure

Antitoxin

Source ID Structure
PDB 3Q8X
PDB 1GVN
AlphaFold DB A0A829F0A3

References