Detailed information of TA system TA224418

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): omega-epsilon-zeta/zeta-epsilon
• Location: 3074827..3075964
• Replicon: chromosome
• Accession: NZ_CP086726
• Organism: Enterococcus faecalis strain E533

Toxin (Protein)

• Gene name: zeta
• Uniprot ID: -
• Locus tag: LM507_RS15000
• Protein ID: WP_002401483.1
• Coordinates: 3075101..3075964 (+)
• Length: 288 a.a.

Antitoxin (Protein)

• Gene name: epsilon
• Uniprot ID: R2XCR7
• Locus tag: LM507_RS14995
• Protein ID: WP_000301765.1
• Coordinates: 3074827..3075099 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM507_RS14960 (3070312)	3070312..3071799	+	1488	WP_010712965.1	type IA DNA topoisomerase	-
LM507_RS14965 (3071919)	3071919..3072041	+	123	Protein_2922	peptide-binding protein	-
LM507_RS14970 (3072110)	3072110..3072205	+	96	WP_001809736.1	23S rRNA methyltransferase attenuation leader peptide	-
LM507_RS14975 (3072330)	3072330..3073067	+	738	WP_002292226.1	23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B)	-
LM507_RS14980 (3073237)	3073237..3073497	+	261	Protein_2925	topoisomerase DNA-binding C4 zinc finger domain-containing protein	-
LM507_RS14985 (3073600)	3073600..3074496	+	897	WP_002387620.1	ParA family protein	-
LM507_RS14990 (3074595)	3074595..3074810	+	216	WP_002295735.1	peptide-binding protein	-
LM507_RS14995 (3074827)	3074827..3075099	+	273	WP_000301765.1	antitoxin	Antitoxin
LM507_RS15000 (3075101)	3075101..3075964	+	864	WP_002401483.1	zeta toxin family protein	Toxin
LM507_RS15005 (3076405)	3076405..3076722	+	318	WP_002333463.1	hypothetical protein	-
LM507_RS15010 (3076742)	3076742..3077029	+	288	Protein_2931	DnaJ domain-containing protein	-
LM507_RS15015 (3077103)	3077103..3077783	+	681	WP_010710189.1	IS6-like element IS1216 family transposase	-
LM507_RS15020 (3077839)	3077839..3078270	+	432	Protein_2933	2-dehydropantoate 2-reductase	-
LM507_RS15025 (3078546)	3078546..3079397	+	852	WP_002357480.1	ribosome biogenesis GTPase YlqF	-
LM507_RS15030 (3079399)	3079399..3080166	+	768	WP_002357481.1	ribonuclease HII	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III / lsa(E) / lnu(B)	-	3047206..3077783	30577

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (27-213)

Antitoxin

• Bacterial epsilon antitoxin (2-90)

Sequences

Toxin

Protein

• Length: 288 a.a.
• Molecular weight: 32486.06 Da
• Isoelectric Point: 6.9965

>T224418 WP_002401483.1 NZ_CP086726:3075101-3075964 [Enterococcus faecalis]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAILEETQGNVIVIDNDTFKQQHPNFDELA
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSRLETPSISPKETLERELNRKVSGKEIQ
PTLERIEQKMLQNQHQETPEFKAIQQKMESLQPPTPPIPKTPKLPGL

Nucleotide

• Length: 864 bp

>T224418 NZ_CP120325:3522071-3522289 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10721.25 Da
• Isoelectric Point: 6.4676

>AT224418 WP_000301765.1 NZ_CP086726:3074827-3075099 [Enterococcus faecalis]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Nucleotide

• Length: 273 bp

>AT224418 NZ_CP120325:3521669-3522043 [Salmonella enterica subsp. enterica serovar Muenchen]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA

Structures

Antitoxin

• AlphaFold DB: A0A829F0A3