Detailed information of TA system TA224417

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 3065284..3066392
• Replicon: chromosome
• Accession: NZ_CP086726
• Organism: Enterococcus faecalis strain E533

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A193AUR0
• Locus tag: LM507_RS14935
• Protein ID: WP_002378351.1
• Coordinates: 3065523..3066392 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: LM507_RS14930
• Protein ID: WP_000205227.1
• Coordinates: 3065284..3065508 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM507_RS14910 (3060915)	3060915..3062399	+	1485	WP_002294513.1	ABC-F type ribosomal protection protein Lsa(E)	-
LM507_RS14915 (3062453)	3062453..3063256	+	804	WP_002294514.1	lincosamide nucleotidyltransferase Lnu(B)	-
LM507_RS14920 (3063565)	3063565..3064866	-	1302	Protein_2913	ISL3 family transposase	-
LM507_RS14925 (3065055)	3065055..3065141	+	87	Protein_2914	site-specific recombinase	-
LM507_RS14930 (3065284)	3065284..3065508	+	225	WP_000205227.1	helix-turn-helix transcriptional regulator	Antitoxin
LM507_RS14935 (3065523)	3065523..3066392	+	870	WP_002378351.1	nucleotidyltransferase domain-containing protein	Toxin
LM507_RS14940 (3066373)	3066373..3067107	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
LM507_RS14945 (3067140)	3067140..3068003	+	864	WP_002294505.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
LM507_RS14950 (3068173)	3068173..3068574	+	402	WP_002401582.1	phosphoribosyltransferase family protein	-
LM507_RS14955 (3068802)	3068802..3070121	+	1320	WP_002338419.1	IS1380-like element ISSsu5 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	IScluster/Tn	erm(B) / aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III / lsa(E) / lnu(B)	-	3047206..3077783	30577
	-	inside	Genomic island	aac(6')-aph(2'') / ant(6)-Ia / aph(3')-III / lsa(E) / lnu(B)	-	3051993..3068574	16581

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-111)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32912.50 Da
• Isoelectric Point: 4.7903

>T224417 WP_002378351.1 NZ_CP086726:3065523-3066392 [Enterococcus faecalis]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
PTALKKSLMNFFIFEAEFSLMFVKANAGVEDKYYIAGHVFRIISCLNQVLFACNNAYCINEKKAIKLLETFEYKPEKYAE
RVNHIFEVLGLSLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T224417 NZ_CP120325:2101792-2101895 [Salmonella enterica subsp. enterica serovar Muenchen]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8365.68 Da
• Isoelectric Point: 4.5510

>AT224417 WP_000205227.1 NZ_CP086726:3065284-3065508 [Enterococcus faecalis]
MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT224417 NZ_CP120325:c2101897-2101752 [Salmonella enterica subsp. enterica serovar Muenchen]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Toxin

• AlphaFold DB: A0A193AUR0