Detailed information of TA system TA224400

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ImmA-IrrE/HTH_19(antitoxin)
• Location: 1401015..1402034
• Replicon: chromosome
• Accession: NZ_CP086726
• Organism: Enterococcus faecalis strain E533

Toxin (Protein)

• Gene name: IrrE
• Uniprot ID: -
• Locus tag: LM507_RS06870
• Protein ID: WP_137274387.1
• Coordinates: 1401396..1402034 (+)
• Length: 213 a.a.

Antitoxin (Protein)

• Gene name: ImmA
• Uniprot ID: -
• Locus tag: LM507_RS06865
• Protein ID: WP_002359284.1
• Coordinates: 1401015..1401389 (+)
• Length: 125 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LM507_RS06820 (1396949)	1396949..1397332	-	384	WP_002359294.1	DUF86 domain-containing protein	-
LM507_RS06825 (1397322)	1397322..1397654	-	333	WP_137274391.1	nucleotidyltransferase domain-containing protein	-
LM507_RS06830 (1397766)	1397766..1398170	-	405	WP_137274390.1	hypothetical protein	-
LM507_RS06835 (1398152)	1398152..1398343	-	192	WP_137274394.1	hypothetical protein	-
LM507_RS06840 (1398372)	1398372..1398596	-	225	WP_137274389.1	hypothetical protein	-
LM507_RS06845 (1398751)	1398751..1399158	-	408	WP_159162087.1	DUF961 family protein	-
LM507_RS06850 (1399158)	1399158..1399484	-	327	WP_002359288.1	hypothetical protein	-
LM507_RS06855 (1399571)	1399571..1399822	-	252	WP_002359287.1	hypothetical protein	-
LM507_RS06860 (1399989)	1399989..1400171	+	183	WP_129217660.1	hypothetical protein	-
LM507_RS06865 (1401015)	1401015..1401389	+	375	WP_002359284.1	helix-turn-helix domain-containing protein	Antitoxin
LM507_RS06870 (1401396)	1401396..1402034	+	639	WP_137274387.1	ImmA/IrrE family metallo-endopeptidase	Toxin
LM507_RS06875 (1402076)	1402076..1402651	-	576	WP_002359282.1	restriction endonuclease	-
LM507_RS06880 (1403361)	1403361..1404716	-	1356	WP_010819693.1	PIN-like domain-containing protein	-
LM507_RS06885 (1405194)	1405194..1406201	-	1008	WP_010819692.1	radical SAM protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	tufA	1335953..1404396	68443

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• IrrE N-terminal-like domain (86-176)

Antitoxin

• Cro/C1-type helix-turn-helix domain (7-61)

Sequences

Toxin

Protein

• Length: 213 a.a.
• Molecular weight: 25202.20 Da
• Isoelectric Point: 9.1676

>T224400 WP_137274387.1 NZ_CP086726:1401396-1402034 [Enterococcus faecalis]
MILNKKKLYFETQAFTDKLINEISINMSKRKELINCYDIEAYVKETENIDFFDYPFKKQSKKLIWGSVTKIGDVTMVATA
QHLKIEIKNFTKMHEIIHHYFDYTTDIPNNEKSFDTLLIKKGYLPEDYPKEFRANKGAGMLLINKKALKYAFSTYDDLEK
MASFFNVNDIVLKIRILDELIYSHNLSYKQANLIYNSYYFQQQTQLKEILFK

Nucleotide

• Length: 639 bp

>T224400 NZ_CP120322:680947-681255 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATACATGGTTTATCGCAATAAAGGAAACAGCAAGGCTTATCCTTATTTACTTGATGTGCAAAGCGATATTATTGA
TGAACTGCATACTCGCATGGTTATCCCATTGTTTCCTGTGAGCAGATTGGTAAATAACCCAGTTAAGCGCCTCACGCCCA
CTCTGAATGTGGAAGGTAATGACTATCTGGTGATGACTCACGAAATGGCGAGTATCAGGCTTTCGCAGATTGGTGATGAG
GTGATGGACGTTCGTTCCCACAGGCAAACCATCAAAAATGCTCTCGATTTTATCTTTGACGGGTTCTGA

Antitoxin

Protein

• Length: 125 a.a.
• Molecular weight: 14518.29 Da
• Isoelectric Point: 4.8896

>AT224400 WP_002359284.1 NZ_CP086726:1401015-1401389 [Enterococcus faecalis]
MKTYEKIKQLRENREITQKEISNALDINVSVYNKIELGIRPLREEELTAIADFFNVSIDYLTGRTDNPTTPSNNQKEAGQ
NIISHFRLNTNDLSSDDIEELEKELIDFQEFLIKRAKERKKKSE

Nucleotide

• Length: 375 bp

>AT224400 NZ_CP120322:680705-680944 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGGGTATGAGTGTACGACATAAAAAAACAGTCAGTGTCACGCTGGAACCTGCATTGCTTCAGCAAGCCCGTGATGC
AGGTATTAATCTGTCAGCGGTGCTTACCGCCGCGCTGAAAGAGGAAATCAGTGCCACAGGCGTTGAACGCTGGAAAGCAG
AAAATAGAGCGGGTTTGCAGGAGCTCAATCGTATTACTGATGAGCACGGCCTTTTATCGGACGATTACAGGACGTTTTAA