Detailed information of TA system TA224392

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 56026..56762
• Replicon: plasmid pKp3b
• Accession: NZ_CP086725
• Organism: Klebsiella pneumoniae strain KP_3

Toxin (Protein)

• Gene name: tacT
• Uniprot ID: A0A8T5ZF70
• Locus tag: LOQ45_RS26015
• Protein ID: WP_004187044.1
• Coordinates: 56280..56762 (+)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: tacA
• Uniprot ID: L7SZ29
• Locus tag: LOQ45_RS26010
• Protein ID: WP_003026799.1
• Coordinates: 56026..56292 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOQ45_RS25995 (pgaptmp_000053)	51498..52892	+	1395	WP_032439652.1	cytosine permease	-
LOQ45_RS26000 (pgaptmp_000054)	52904..54112	+	1209	WP_032439650.1	imidazolonepropionase	-
LOQ45_RS26005 (pgaptmp_000055)	54105..54914	+	810	WP_023329017.1	N-formylglutamate deformylase	-
LOQ45_RS26010 (pgaptmp_000057)	56026..56292	+	267	WP_003026799.1	DUF1778 domain-containing protein	Antitoxin
LOQ45_RS26015 (pgaptmp_000058)	56280..56762	+	483	WP_004187044.1	GNAT family N-acetyltransferase	Toxin
LOQ45_RS26020 (pgaptmp_000059)	56969..58315	+	1347	WP_077254728.1	ISNCY family transposase	-
LOQ45_RS26025 (pgaptmp_000060)	58364..58762	+	399	WP_032422684.1	helix-turn-helix domain-containing protein	-
LOQ45_RS26030 (pgaptmp_000061)	58909..59019	-	111	Protein_58	IS3 family transposase	-
LOQ45_RS26035 (pgaptmp_000063)	59294..60230	+	937	Protein_59	ISNCY family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IIa / blaOXA-1 / aac(6')-Ib-cr / blaCTX-M-15 / blaTEM-1B / aph(6)-Id / aph(3'')-Ib / sul2 / dfrA14 / qnrB1 / tet(A)	-	1..230544	230544

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (46-148)

Antitoxin

• Vibrio phage ICP1, Orf50 (6-82)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17280.93 Da
• Isoelectric Point: 8.7197

>T224392 WP_004187044.1 NZ_CP086725:56280-56762 [Klebsiella pneumoniae]
VGCITAPEPLSSAHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTYERTLFLKLP

Nucleotide

• Length: 483 bp

>T224392 NZ_CP120318:c4529372-4529061 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGAGGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10147.59 Da
• Isoelectric Point: 4.5256

>AT224392 WP_003026799.1 NZ_CP086725:56026-56292 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Nucleotide

• Length: 267 bp

>AT224392 NZ_CP120318:c4529060-4528770 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAGCCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAAC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A1Q8YL66