Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5253390..5254015 Replicon chromosome
Accession NZ_CP086724
Organism Klebsiella pneumoniae strain KP_3

Toxin (Protein)


Gene name vapC Uniprot ID A0A378FVD4
Locus tag LOQ45_RS25375 Protein ID WP_019705794.1
Coordinates 5253390..5253773 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag LOQ45_RS25380 Protein ID WP_004150355.1
Coordinates 5253773..5254015 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOQ45_RS25360 (5250756) 5250756..5251658 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
LOQ45_RS25365 (5251655) 5251655..5252290 + 636 WP_032430299.1 formate dehydrogenase cytochrome b556 subunit -
LOQ45_RS25370 (5252287) 5252287..5253216 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
LOQ45_RS25375 (5253390) 5253390..5253773 - 384 WP_019705794.1 type II toxin-antitoxin system VapC family toxin Toxin
LOQ45_RS25380 (5253773) 5253773..5254015 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
LOQ45_RS25385 (5254220) 5254220..5255137 + 918 WP_032430300.1 alpha/beta hydrolase -
LOQ45_RS25390 (5255152) 5255152..5256093 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
LOQ45_RS25395 (5256138) 5256138..5256575 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
LOQ45_RS25400 (5256572) 5256572..5257432 - 861 WP_004146232.1 virulence factor BrkB family protein -
LOQ45_RS25405 (5257426) 5257426..5258025 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14358.57 Da        Isoelectric Point: 6.8806

>T224390 WP_019705794.1 NZ_CP086724:c5253773-5253390 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGHREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224390 NZ_CP120318:c4113024-4112740 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224390 WP_004150355.1 NZ_CP086724:c5254015-5253773 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224390 NZ_CP120318:c4113256-4113014 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A378FVD4

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References