Detailed information of TA system TA224387

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): ykfI-yfjZ/CbtA-CbeA
• Location: 4694051..4694743
• Replicon: chromosome
• Accession: NZ_CP086724
• Organism: Klebsiella pneumoniae strain KP_3

Toxin (Protein)

• Gene name: ykfI
• Uniprot ID: -
• Locus tag: LOQ45_RS22765
• Protein ID: WP_071603219.1
• Coordinates: 4694051..4694392 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: yfjZ
• Uniprot ID: -
• Locus tag: LOQ45_RS22770
• Protein ID: WP_040189932.1
• Coordinates: 4694417..4694743 (-)
• Length: 109 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOQ45_RS22740 (4689959)	4689959..4691413	-	1455	WP_074184809.1	serine protease	-
LOQ45_RS22745 (4691601)	4691601..4692398	-	798	WP_038806919.1	helix-turn-helix transcriptional regulator	-
LOQ45_RS22750 (4692499)	4692499..4692774	-	276	Protein_4463	type II toxin-antitoxin system RelE/ParE family toxin	-
LOQ45_RS22755 (4692778)	4692778..4693026	-	249	WP_038806909.1	ribbon-helix-helix domain-containing protein	-
LOQ45_RS22760 (4693103)	4693103..4693936	-	834	WP_038806910.1	DUF4942 domain-containing protein	-
LOQ45_RS22765 (4694051)	4694051..4694392	-	342	WP_071603219.1	TA system toxin CbtA family protein	Toxin
LOQ45_RS22770 (4694417)	4694417..4694743	-	327	WP_040189932.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
LOQ45_RS22775 (4694757)	4694757..4695233	-	477	WP_038806912.1	DNA repair protein RadC	-
LOQ45_RS22780 (4695244)	4695244..4695690	-	447	WP_038806913.1	antirestriction protein	-
LOQ45_RS22785 (4695914)	4695914..4696603	-	690	WP_038806914.1	hypothetical protein	-
LOQ45_RS22790 (4696922)	4696922..4697275	+	354	WP_038806915.1	hypothetical protein	-
LOQ45_RS22795 (4697312)	4697312..4698202	-	891	WP_038806917.1	YfjP family GTPase	-
LOQ45_RS22800 (4698300)	4698300..4699565	+	1266	WP_166686293.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4677555..4716932	39377

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-107)

Antitoxin

• Antitoxin CbeA (8-107)

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 12762.63 Da
• Isoelectric Point: 7.8846

>T224387 WP_071603219.1 NZ_CP086724:c4694392-4694051 [Klebsiella pneumoniae]
MKTIPATTQRAAKSCPSPVAFWQMPLTRLLEQHYGLTLNDTPFSDETAIQEHINAGITLVDAVNFLVEKYELVRIDRRGF
NCQEQSPYLGAVDILRARQVTGLLRQSPHPSIR

Nucleotide

• Length: 342 bp

>T224387 NZ_CP120318:3636567-3636824 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin

Protein

• Length: 109 a.a.
• Molecular weight: 12333.06 Da
• Isoelectric Point: 6.9560

>AT224387 WP_040189932.1 NZ_CP086724:c4694743-4694417 [Klebsiella pneumoniae]
MPLTDAIQWGLKRSFTPNFGARLVQEGNRLHYLADRANITGKFVDAECQKLEEAFPHFIRQMELMLSTGELNPRHAHSVT
LYHNAFTCEADTLGSCGYVYIVIYPTQR

Nucleotide

• Length: 327 bp

>AT224387 NZ_CP120318:c3636742-3636410 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT