Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4650611..4651421 Replicon chromosome
Accession NZ_CP086724
Organism Klebsiella pneumoniae strain KP_3

Toxin (Protein)


Gene name KacT Uniprot ID A0A060VJ83
Locus tag LOQ45_RS22560 Protein ID WP_004178461.1
Coordinates 4650611..4651144 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag LOQ45_RS22565 Protein ID WP_002887278.1
Coordinates 4651155..4651421 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOQ45_RS22555 (4649442) 4649442..4650563 + 1122 WP_021441121.1 cupin domain-containing protein -
LOQ45_RS22560 (4650611) 4650611..4651144 - 534 WP_004178461.1 type II toxin-antitoxin system toxin KacT Toxin
LOQ45_RS22565 (4651155) 4651155..4651421 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
LOQ45_RS22570 (4651524) 4651524..4652957 - 1434 WP_021441120.1 Cu(+)/Ag(+) sensor histidine kinase -
LOQ45_RS22575 (4652947) 4652947..4653630 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
LOQ45_RS22580 (4653803) 4653803..4655188 + 1386 WP_038806759.1 efflux transporter outer membrane subunit -
LOQ45_RS22585 (4655206) 4655206..4655550 + 345 WP_021441118.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19810.65 Da        Isoelectric Point: 5.2614

>T224386 WP_004178461.1 NZ_CP086724:c4651144-4650611 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T224386 NZ_CP120318:3446206-3446424 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT224386 WP_002887278.1 NZ_CP086724:c4651421-4651155 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT224386 NZ_CP120318:3445804-3446178 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A060VJ83

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References