Detailed information of TA system TA224384

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2669091..2670679
• Replicon: chromosome
• Accession: NZ_CP086724
• Organism: Klebsiella pneumoniae strain KP_3

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A6T928
• Locus tag: LOQ45_RS13005
• Protein ID: WP_004176197.1
• Coordinates: 2669091..2670413 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LOQ45_RS13010
• Protein ID: WP_032417111.1
• Coordinates: 2670413..2670679 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOQ45_RS12990 (2664147)	2664147..2666648	+	2502	WP_016831617.1	fimbria/pilus outer membrane usher protein	-
LOQ45_RS12995 (2666636)	2666636..2667640	+	1005	WP_004176196.1	fimbrial protein	-
LOQ45_RS13000 (2667782)	2667782..2669074	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
LOQ45_RS13005 (2669091)	2669091..2670413	-	1323	WP_004176197.1	type II toxin-antitoxin system HipA family toxin	Toxin
LOQ45_RS13010 (2670413)	2670413..2670679	-	267	WP_032417111.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LOQ45_RS13015 (2670944)	2670944..2671270	-	327	WP_002904788.1	hydroxyisourate hydrolase	-
LOQ45_RS13020 (2671267)	2671267..2671767	-	501	WP_002904787.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
LOQ45_RS13025 (2671745)	2671745..2673124	-	1380	WP_117259192.1	nucleobase:cation symporter-2 family protein	-
LOQ45_RS13030 (2673470)	2673470..2674624	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
LOQ45_RS13035 (2674605)	2674605..2675528	-	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49111.41 Da
• Isoelectric Point: 7.5357

>T224384 WP_004176197.1 NZ_CP086724:c2670413-2669091 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITGQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T224384 NZ_CP120318:c2478248-2478093 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9926.32 Da
• Isoelectric Point: 8.5912

>AT224384 WP_032417111.1 NZ_CP086724:c2670679-2670413 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETASAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT224384 NZ_CP120318:2478260-2478318 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Structures

Toxin

• AlphaFold DB: A6T928