Detailed information of TA system TA224372

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 344615..345201
• Replicon: chromosome
• Accession: NZ_CP086724
• Organism: Klebsiella pneumoniae strain KP_3

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A331ME34
• Locus tag: LOQ45_RS01590
• Protein ID: WP_025861226.1
• Coordinates: 344833..345201 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: LOQ45_RS01585
• Protein ID: WP_004174006.1
• Coordinates: 344615..344836 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LOQ45_RS01565 (340771)	340771..341697	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
LOQ45_RS01570 (341694)	341694..342971	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
LOQ45_RS01575 (342968)	342968..343735	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
LOQ45_RS01580 (343737)	343737..344450	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
LOQ45_RS01585 (344615)	344615..344836	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
LOQ45_RS01590 (344833)	344833..345201	+	369	WP_025861226.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
LOQ45_RS01595 (345474)	345474..346790	+	1317	WP_004174008.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
LOQ45_RS01600 (346897)	346897..347784	+	888	WP_014906831.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
LOQ45_RS01605 (347781)	347781..348626	+	846	WP_004145129.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
LOQ45_RS01610 (348628)	348628..349698	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	304122..350435	46313

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13551.98 Da
• Isoelectric Point: 8.6410

>T224372 WP_025861226.1 NZ_CP086724:344833-345201 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGIKRTALF
ITLLFLKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T224372 NZ_CP120315:c4113017-4112733 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGACTTATGAACTGGAATTCGACCCGAGGGCCTTAAAAGAGTGGCATAAGCTGGGCGATACGGTGCAGGCTCAGCTTAA
GAAAAAGTTGGCTGATGTGCTATTGAACCCCAGAATCGACTCTGCCCGTTTAAACGATCTTCCTGACTGCTATAAAATTA
AGCTTAAATCGTCCGGTTATCGCTTGGTGTACCAGGTTCGGGATGACGTTGTGATTGTGTTTGTTGTCGCGGTCGGTAAG
CGAGAACATTCAGCCGTCTATCACGATGCAAACAAACGGCTTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT224372 WP_004174006.1 NZ_CP086724:344615-344836 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT224372 NZ_CP120315:c4113249-4113007 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGCCACGCTGAACGTCCGTCTGGATGACAAACTCAAAAATGAGGCTTATGCCGTGCTGGAAAAACTGAACATTACTCC
AACGGAAGCCGTGCGATTACTGTTCCAGTACGTCGCGGAAACGGGGCGCATGCCAGTGAAAACCGTCACTCTTAGCGACA
GTGAAGATGCATTGATTCAGACAGTTCGGGAGAGGCTGTCTAGTCCGCAAAAGGGAATCAAGGTTAGTCTGGATGACTTA
TGA

Structures

Toxin

• AlphaFold DB: A0A331ME34

Antitoxin

• AlphaFold DB: A0A5E5YJY7