Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/Doc-Phd
Location 22082..22683 Replicon plasmid pMADE-3
Accession NZ_CP086681
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name doc Uniprot ID V0AJ64
Locus tag LMF34_RS26330 Protein ID WP_001216034.1
Coordinates 22303..22683 (+) Length 127 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID U9YQH9
Locus tag LMF34_RS26325 Protein ID WP_001190712.1
Coordinates 22082..22303 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS26300 18088..19068 + 981 WP_072649545.1 IS5-like element ISKpn26 family transposase -
LMF34_RS26310 20842..21189 + 348 WP_072716716.1 IS66 family insertion sequence element accessory protein TnpB -
LMF34_RS26315 21239..21445 + 207 Protein_23 IS66 family transposase -
LMF34_RS26320 21620..22009 + 390 WP_000506730.1 S24 family peptidase -
LMF34_RS26325 22082..22303 + 222 WP_001190712.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
LMF34_RS26330 22303..22683 + 381 WP_001216034.1 type II toxin-antitoxin system death-on-curing family toxin Toxin
LMF34_RS26335 22688..22867 + 180 WP_000113018.1 hypothetical protein -
LMF34_RS26340 22895..23938 + 1044 WP_248422989.1 DUF968 domain-containing protein -
LMF34_RS26345 24027..24479 + 453 WP_097307769.1 Late promoter-activating protein -
LMF34_RS26350 24566..25714 + 1149 WP_248422988.1 terminase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid mph(A) - 1..135562 135562
- inside IScluster/Tn - - 18088..29156 11068


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Death on curing protein

(2-123)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-51)


Sequences

Toxin        


Download         Length: 127 a.a.        Molecular weight: 13615.32 Da        Isoelectric Point: 5.1514

>T224370 WP_001216034.1 NZ_CP086681:22303-22683 [Escherichia coli]
MRHISPEELIALHDANINRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVADTLRRLYGSAE

Download         Length: 381 bp

>T224370 NZ_CP120315:c4021903-4021616 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGCCGATGGAGTTTGAATGGGATGCGAACAAGGCTAAAAGCAACCTGAGGAAGCACGGTGTCCGCTTTGAGGATGCGGT
ACTGGTGTTTGACGATCCCCGACACCTGTCACGTCAGGAGCGTTATGAGAATGGAGAATATCGTTGGCAGACCCTCGGCC
TTGTTCATGGCATTGTGGTCATTCTGGTTGCTCATAGCGTTCGGTTCGAAAGTGGTTTTGAAGTTATCCGTATCATCAGC
GCTCGCAAGGCAGACAGAAAAGAGAGGAATCGTTATGAGCATGGTTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 8133.10 Da        Isoelectric Point: 4.7708

>AT224370 WP_001190712.1 NZ_CP086681:22082-22303 [Escherichia coli]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Download         Length: 222 bp

>AT224370 NZ_CP120315:c4021629-4021327 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAGCATGGTTAAACATAAACGCGGCAATGCATCCGCGCTGAGTGCTCAGCATGAGGCTGAACTGAAGGCGCTGGCGAA
AAAATCAGATGATGAGATTGATTACAGCGACATTCCCGCTTCGGAGGATGGGCAGTGGTCTGAAGCAGTGCGTGGTAAGT
TTTTTCGCCCGTTAAAAACTCAGGCCTCGGTGCGTATTGACGCCGATGTGATGGAGTGGCTGAAACGGCCAGGAAAAGGC
TACCAGACGCGGCTCAATGCCATTCTGCGCGAGGCGATGCTGCGCGAGCAAAATAAGAAATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0AJ64

Antitoxin

Source ID Structure
AlphaFold DB A0A829CJB6

References