Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4376606..4377103 Replicon chromosome
Accession NC_009800
Organism Escherichia coli HS

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag ECHS_RS21520 Protein ID WP_001173343.1
Coordinates 4376930..4377103 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID J7RC38
Locus tag ECHS_RS21515 Protein ID WP_001425728.1
Coordinates 4376606..4376902 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHS_RS21495 (EcHS_A4365) 4373337..4374056 - 720 WP_000611288.1 two-component system response regulator DcuR -
ECHS_RS21500 (EcHS_A4366) 4374053..4375684 - 1632 WP_001216486.1 sensor histidine kinase -
ECHS_RS21505 (EcHS_A4367) 4375865..4376095 + 231 WP_000371698.1 (4Fe-4S)-binding protein -
ECHS_RS21510 (EcHS_A4368) 4376107..4376379 + 273 WP_000405647.1 N-acetyltransferase -
ECHS_RS21515 4376606..4376902 + 297 WP_001425728.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
ECHS_RS21520 (EcHS_A4369) 4376930..4377103 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
ECHS_RS21525 (EcHS_A4370) 4377222..4378739 - 1518 WP_001295074.1 lysine--tRNA ligase -
ECHS_RS21530 (EcHS_A4371) 4378976..4380433 - 1458 WP_000856829.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T22437 WP_001173343.1 NC_009800:4376930-4377103 [Escherichia coli HS]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T22437 NC_009800:4376930-4377103 [Escherichia coli HS]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11466.99 Da        Isoelectric Point: 4.2544

>AT22437 WP_001425728.1 NC_009800:4376606-4376902 [Escherichia coli HS]
MEGKNKFNTYVVSFNYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT22437 NC_009800:4376606-4376902 [Escherichia coli HS]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTAATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
AlphaFold DB J7RC38

References