Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) relBE/ParE-DnaT
Location 32881..33403 Replicon plasmid pMADE-2
Accession NZ_CP086680
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name relE Uniprot ID G3CAN5
Locus tag LMF34_RS26105 Protein ID WP_000220560.1
Coordinates 32881..33162 (-) Length 94 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID G3CAG1
Locus tag LMF34_RS26110 Protein ID WP_000121743.1
Coordinates 33152..33403 (-) Length 84 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS26065 27970..29082 + 1113 WP_249469359.1 class 1 integron integrase IntI1 -
LMF34_RS26070 29028..29734 - 707 Protein_42 IS6-like element IS26 family transposase -
LMF34_RS26075 29802..30038 + 237 Protein_43 Tn3 family transposase -
LMF34_RS26080 30154..30342 + 189 WP_000957857.1 hypothetical protein -
LMF34_RS26085 30352..31553 + 1202 Protein_45 IS91 family transposase -
LMF34_RS26090 31917..32132 - 216 WP_001180116.1 hypothetical protein -
LMF34_RS26095 32122..32367 - 246 WP_248422970.1 hypothetical protein -
LMF34_RS26100 32412..32735 - 324 WP_001181903.1 hypothetical protein -
LMF34_RS26105 32881..33162 - 282 WP_000220560.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
LMF34_RS26110 33152..33403 - 252 WP_000121743.1 plasmid stabilization protein Antitoxin
LMF34_RS26115 34659..35495 + 837 WP_001050931.1 RepB family plasmid replication initiator protein -
LMF34_RS26120 35535..35981 + 447 WP_001074386.1 hypothetical protein -
LMF34_RS26125 36118..36471 + 354 WP_000876434.1 DNA distortion polypeptide 3 -
LMF34_RS26130 36531..37499 - 969 WP_077249520.1 IS5-like element IS903B family transposase -
LMF34_RS26135 37501..37794 - 294 WP_015060510.1 hypothetical protein -
LMF34_RS26140 37810..38040 - 231 WP_000051064.1 plasmid partition protein ParG -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1 - 1..46324 46324
- inside IScluster/Tn tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1 - 1..31429 31428


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-86)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 94 a.a.        Molecular weight: 11005.83 Da        Isoelectric Point: 10.5938

>T224369 WP_000220560.1 NZ_CP086680:c33162-32881 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Download         Length: 282 bp

>T224369 NZ_CP120315:3636561-3636818 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9194.43 Da        Isoelectric Point: 4.1683

>AT224369 WP_000121743.1 NZ_CP086680:c33403-33152 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Download         Length: 252 bp

>AT224369 NZ_CP120315:c3636736-3636404 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB G3CAN5


Antitoxin

Source ID Structure
AlphaFold DB A0A3S5I301

References