Detailed information of TA system
Bioinformatically predictedOverview
TA module
Type | II | Classification (family/domain) | relBE/ParE-DnaT |
Location | 32881..33403 | Replicon | plasmid pMADE-2 |
Accession | NZ_CP086680 | ||
Organism | Escherichia coli strain EFF60 |
Toxin (Protein)
Gene name | relE | Uniprot ID | G3CAN5 |
Locus tag | LMF34_RS26105 | Protein ID | WP_000220560.1 |
Coordinates | 32881..33162 (-) | Length | 94 a.a. |
Antitoxin (Protein)
Gene name | relB | Uniprot ID | G3CAG1 |
Locus tag | LMF34_RS26110 | Protein ID | WP_000121743.1 |
Coordinates | 33152..33403 (-) | Length | 84 a.a. |
Genomic Context
Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
---|---|---|---|---|---|---|
LMF34_RS26065 | 27970..29082 | + | 1113 | WP_249469359.1 | class 1 integron integrase IntI1 | - |
LMF34_RS26070 | 29028..29734 | - | 707 | Protein_42 | IS6-like element IS26 family transposase | - |
LMF34_RS26075 | 29802..30038 | + | 237 | Protein_43 | Tn3 family transposase | - |
LMF34_RS26080 | 30154..30342 | + | 189 | WP_000957857.1 | hypothetical protein | - |
LMF34_RS26085 | 30352..31553 | + | 1202 | Protein_45 | IS91 family transposase | - |
LMF34_RS26090 | 31917..32132 | - | 216 | WP_001180116.1 | hypothetical protein | - |
LMF34_RS26095 | 32122..32367 | - | 246 | WP_248422970.1 | hypothetical protein | - |
LMF34_RS26100 | 32412..32735 | - | 324 | WP_001181903.1 | hypothetical protein | - |
LMF34_RS26105 | 32881..33162 | - | 282 | WP_000220560.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
LMF34_RS26110 | 33152..33403 | - | 252 | WP_000121743.1 | plasmid stabilization protein | Antitoxin |
LMF34_RS26115 | 34659..35495 | + | 837 | WP_001050931.1 | RepB family plasmid replication initiator protein | - |
LMF34_RS26120 | 35535..35981 | + | 447 | WP_001074386.1 | hypothetical protein | - |
LMF34_RS26125 | 36118..36471 | + | 354 | WP_000876434.1 | DNA distortion polypeptide 3 | - |
LMF34_RS26130 | 36531..37499 | - | 969 | WP_077249520.1 | IS5-like element IS903B family transposase | - |
LMF34_RS26135 | 37501..37794 | - | 294 | WP_015060510.1 | hypothetical protein | - |
LMF34_RS26140 | 37810..38040 | - | 231 | WP_000051064.1 | plasmid partition protein ParG | - |
Associated MGEs
MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
---|---|---|---|---|---|---|---|
- | inside | Non-Mobilizable plasmid | tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1 | - | 1..46324 | 46324 | |
- | inside | IScluster/Tn | tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1 | - | 1..31429 | 31428 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 94 a.a. Molecular weight: 11005.83 Da Isoelectric Point: 10.5938
>T224369 WP_000220560.1 NZ_CP086680:c33162-32881 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR
Download Length: 282 bp
>T224369 NZ_CP120315:3636561-3636818 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCAAAACCCACATGACCACCTATTACTCACAAATCCCCAGCCT
GCATCTTAAGGGCGACTGGCTGGAAGAAGCGGGATTTAAGACCGGGCGCGGCGTCACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAGCAGCTTTATCAGGCTAAACAGGTGGTTAAGGGAATT
AAGGACGTGCTGGTTTAG
Antitoxin
Download Length: 84 a.a. Molecular weight: 9194.43 Da Isoelectric Point: 4.1683
>AT224369 WP_000121743.1 NZ_CP086680:c33403-33152 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL
Download Length: 252 bp
>AT224369 NZ_CP120315:c3636736-3636404 [Salmonella enterica subsp. enterica serovar Enteritidis]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTGACGCCGCGCCCGGTCTTAAATCCCGCTTCTTCCAGCCA
GTCGCCCTTAAGATGCAGGCTGGGGATTTGTGAGTAATAGGTGGTCATGTGGGTTTTGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTATCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACTGAGCCCCCCTCTCCCCGGCCAGCTCCGGGCGGTGATGAGCAACCGGCTGCC
GGGGCCGTATTTT
Similar Proteins
Only experimentally validated proteins are listed.
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
---|
Structures
Toxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | G3CAN5 |
Antitoxin
Source | ID | Structure |
---|---|---|
AlphaFold DB | A0A3S5I301 |