Detailed information of TA system TA224368

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-DnaT
• Location: 10520..11042
• Replicon: plasmid pMADE-2
• Accession: NZ_CP086680
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: relE
• Uniprot ID: G3CAN5
• Locus tag: LMF34_RS25935
• Protein ID: WP_000220560.1
• Coordinates: 10520..10801 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: G3CAG1
• Locus tag: LMF34_RS25940
• Protein ID: WP_000121743.1
• Coordinates: 10791..11042 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS25895	5613..6725	+	1113	WP_024166810.1	class 1 integron integrase IntI1	-
LMF34_RS25900	6671..7375	-	705	WP_001067855.1	IS6-like element IS26 family transposase	-
LMF34_RS25905	7443..7679	+	237	Protein_9	Tn3 family transposase	-
LMF34_RS25910	7795..7983	+	189	WP_000957857.1	hypothetical protein	-
LMF34_RS25915	7993..9192	+	1200	WP_000948429.1	IS91 family transposase	-
LMF34_RS25920	9556..9771	-	216	WP_001180116.1	hypothetical protein	-
LMF34_RS25925	9761..10006	-	246	WP_248422970.1	hypothetical protein	-
LMF34_RS25930	10051..10374	-	324	WP_001181903.1	hypothetical protein	-
LMF34_RS25935	10520..10801	-	282	WP_000220560.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LMF34_RS25940	10791..11042	-	252	WP_000121743.1	plasmid stabilization protein	Antitoxin
LMF34_RS25945	12298..13134	+	837	WP_001050931.1	RepB family plasmid replication initiator protein	-
LMF34_RS25950	13174..13620	+	447	WP_001074386.1	hypothetical protein	-
LMF34_RS25955	13757..14110	+	354	WP_000876434.1	DNA distortion polypeptide 3	-
LMF34_RS25960	14170..15138	-	969	WP_077249520.1	IS5-like element IS903B family transposase	-
LMF34_RS25965	15140..15433	-	294	WP_015060510.1	hypothetical protein	-
LMF34_RS25970	15449..15679	-	231	WP_000051064.1	plasmid partition protein ParG	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1	-	1..46324	46324
	-	inside	IScluster/Tn	tet(A) / floR / dfrA14 / blaTEM-176 / qnrS1	-	1..31429	31428

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-86)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 11005.83 Da
• Isoelectric Point: 10.5938

>T224368 WP_000220560.1 NZ_CP086680:c10801-10520 [Escherichia coli]
MTYELAFDRRALKEWQKLGHTIREQFKKKLAERLENPRVPAARLHGHADRYKIKLRASGYRLVYQVIDEKVVLLVIAVGR
RESSEVYQIADLR

Nucleotide

• Length: 282 bp

>T224368 NZ_CP120315:3446201-3446419 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCCGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9194.43 Da
• Isoelectric Point: 4.1683

>AT224368 WP_000121743.1 NZ_CP086680:c11042-10791 [Escherichia coli]
MSYQILTTTAASITDLKKNPMGTVAEGEGDAVAILNRNEPAFYCVPPKLYAYYRELAEDAELNAVADERMKNPEIVKVNL
DDL

Nucleotide

• Length: 252 bp

>AT224368 NZ_CP120315:3445799-3446173 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Structures

Toxin

• AlphaFold DB: G3CAN5

Antitoxin

• AlphaFold DB: A0A3S5I301