Detailed information of TA system TA224367

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 25314..26937
• Replicon: plasmid pMADE-1
• Accession: NZ_CP086679
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: LMF34_RS24600
• Protein ID: WP_086722013.1
• Coordinates: 25314..26639 (-)
• Length: 442 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A5Z6V2D1
• Locus tag: LMF34_RS24605
• Protein ID: WP_024136327.1
• Coordinates: 26644..26937 (-)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS24570	21984..22283	-	300	WP_001572440.1	hypothetical protein	-
LMF34_RS24575	22338..22574	-	237	Protein_25	type I restriction-modification system subunit M N-terminal domain-containing protein	-
LMF34_RS24580	23028..23453	-	426	WP_001043843.1	hypothetical protein	-
LMF34_RS24585	23707..24522	-	816	WP_001572439.1	HNH endonuclease signature motif containing protein	-
LMF34_RS24590	24535..24945	-	411	WP_000985911.1	hypothetical protein	-
LMF34_RS24595	25047..25253	-	207	WP_000134171.1	hypothetical protein	-
LMF34_RS24600	25314..26639	-	1326	WP_086722013.1	type II toxin-antitoxin system HipA family toxin	Toxin
LMF34_RS24605	26644..26937	-	294	WP_024136327.1	helix-turn-helix transcriptional regulator	Antitoxin
LMF34_RS24610	27541..27933	-	393	WP_000341066.1	hypothetical protein	-
LMF34_RS24615	28306..28647	+	342	WP_001151575.1	hypothetical protein	-
LMF34_RS24620	28739..28930	+	192	WP_000398480.1	hypothetical protein	-
LMF34_RS24625	28980..29261	+	282	WP_000077926.1	hypothetical protein	-
LMF34_RS26940	29602..29733	-	132	WP_000374059.1	hypothetical protein	-
LMF34_RS24630	29824..31065	-	1242	WP_001053338.1	VWA domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	sul1 / blaCTX-M-14 / fosA3 / aac(3)-IVa / sul2 / floR	-	1..262144	262144

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (9-108)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-69)

Sequences

Toxin

Protein

• Length: 442 a.a.
• Molecular weight: 50263.22 Da
• Isoelectric Point: 6.1932

>T224367 WP_086722013.1 NZ_CP086679:c26639-25314 [Escherichia coli]
MSRKQQRLVIWMNGIKVGYWEKSKGVDSLEYLPEWVADEQGRPLSLSLPFTPGNQVWRGNVVRDYFDNLLPDSEGIRRRL
AMRYKADSLEPFDLLTELGKDCVGAIQLLHDGDEPTDLYSVKYHPLTESEIAATLRNTTETLLPGRPEDNDDLRLSIAGA
QEKTALLWHEERWCMPEGNTPTTHIFKLPLGLVGNMKADMSSSVENEWLCSVLLGQYGLPVARTQIAHFEDQKALVVERF
DRKWSGDGQWIIRLPQEDMCQALGVSPLRKYQADGGPGISEIMEVLSNSDRAERDKAQFFMTQIIFWMMAATDGHAKNFS
ISIGPQGRYHLTPNYDVLSAWPVIGHGNNQISWQKCKLAMAVRGSSNYYQIYRIQRRHWIRHGEITGLSKQQTEAMIEEI
IARTPGVIERVSGLLPDQFPQQLAESIFDGMRQQCRRLAEK

Nucleotide

• Length: 1326 bp

>T224367 NZ_CP120315:c2727709-2727606 [Salmonella enterica subsp. enterica serovar Enteritidis]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 10946.35 Da
• Isoelectric Point: 10.0925

>AT224367 WP_024136327.1 NZ_CP086679:c26937-26644 [Escherichia coli]
INNDYPLNTLNQLRPLLIGFRKANGLTQKDLSERLGVTQQTYSRLEANPASASIERLFKVFSILGVKISFSSATTSSERK
QTEEMLKSNSPARQEDW

Nucleotide

• Length: 294 bp

>AT224367 NZ_CP120315:2727604-2727749 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTGCG

Structures

Antitoxin

• AlphaFold DB: A0A5Z6V2D1