Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 4649350..4649988 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name hicA Uniprot ID A0A0D8WF76
Locus tag LMF34_RS22310 Protein ID WP_001306887.1
Coordinates 4649812..4649988 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag LMF34_RS22305 Protein ID WP_001270286.1
Coordinates 4649350..4649766 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS22285 (4644502) 4644502..4645443 - 942 WP_001251315.1 ABC transporter permease -
LMF34_RS22290 (4645444) 4645444..4646457 - 1014 WP_001523231.1 ABC transporter ATP-binding protein -
LMF34_RS22295 (4646475) 4646475..4647620 - 1146 WP_020233879.1 ABC transporter substrate-binding protein -
LMF34_RS22300 (4647865) 4647865..4649271 - 1407 WP_001523229.1 PLP-dependent aminotransferase family protein -
LMF34_RS22305 (4649350) 4649350..4649766 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
LMF34_RS22310 (4649812) 4649812..4649988 - 177 WP_001306887.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
LMF34_RS22315 (4650210) 4650210..4650440 + 231 WP_000494241.1 YncJ family protein -
LMF34_RS22320 (4650532) 4650532..4652493 - 1962 WP_024166512.1 23S rRNA 5-hydroxycytidine C2501 synthase -
LMF34_RS22325 (4652566) 4652566..4653102 - 537 WP_020233877.1 DNA-binding transcriptional regulator SutR -
LMF34_RS22330 (4653194) 4653194..4654315 + 1122 WP_248422948.1 benzoate/H(+) symporter BenE family transporter -
LMF34_RS22335 (4654423) 4654423..4654881 - 459 WP_000526135.1 IS200/IS605-like element IS200C family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4654423..4654881 458


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6768.88 Da        Isoelectric Point: 11.5336

>T224366 WP_001306887.1 NZ_CP086678:c4649988-4649812 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFRGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T224366 NZ_CP120315:c2478246-2478091 [Salmonella enterica subsp. enterica serovar Enteritidis]
ATGAAGCAGCAAAAGGCGATGTTAATCGCCCTGATCGTCATCTGTTTAACCGTCATAGTGACGGCACTGGTAACGAGGAA
AGACCTCTGCGAGGTACGAATCCGAACCGGCCAGACGGAGGTCGCTGTCTTCACAGCTTACGAACCTGAGGAGTAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT224366 WP_001270286.1 NZ_CP086678:c4649766-4649350 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT224366 NZ_CP120315:2478258-2478316 [Salmonella enterica subsp. enterica serovar Enteritidis]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGCAAAGCATGAAATTGGCCTCA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0D8WF76

Antitoxin

Source ID Structure

References