Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2979008..2979662 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LMF34_RS14250 Protein ID WP_000244781.1
Coordinates 2979255..2979662 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LMF34_RS14245 Protein ID WP_000354046.1
Coordinates 2979008..2979274 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS14225 (2975096) 2975096..2976529 - 1434 WP_001521235.1 6-phospho-beta-glucosidase BglA -
LMF34_RS14230 (2976574) 2976574..2976885 + 312 WP_001182949.1 N(4)-acetylcytidine aminohydrolase -
LMF34_RS14235 (2977049) 2977049..2977708 + 660 WP_000250269.1 hemolysin III family protein -
LMF34_RS14240 (2977785) 2977785..2978765 - 981 WP_000886050.1 tRNA-modifying protein YgfZ -
LMF34_RS14245 (2979008) 2979008..2979274 + 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LMF34_RS14250 (2979255) 2979255..2979662 + 408 WP_000244781.1 protein YgfX Toxin
LMF34_RS14255 (2979702) 2979702..2980223 - 522 WP_001521233.1 flavodoxin FldB -
LMF34_RS14260 (2980335) 2980335..2981231 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LMF34_RS14265 (2981256) 2981256..2981966 + 711 WP_001521231.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LMF34_RS14270 (2981972) 2981972..2983705 + 1734 WP_021523238.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224352 WP_000244781.1 NZ_CP086678:2979255-2979662 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224352 NZ_CP120211:c2042116-2041832 [Agrobacterium leguminum]
ATGCAGCAAAAGGGCGCGATTGCTCTCGGCTGGAGCCATCAGGGCTGGGAAGACTATCTTCACTGGCAGCAATATGATCG
CAAGATGCTCGAACGCATCAATGAACTCATCAAGGATTGTCGACGGCATCCATTTGAGGGGATCGGAAAACCTGAGCCAC
TGCGTTTTCAGTTGAAGGGTCTATGGTCACGCAGGATTTCGCAGGAGCATCGGCTTGTCTACGAGTTGCGCGGCGAAGCG
GAAAAGACGGTTCTGATTATTCACCAATGCCGTTTCCACTACTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT224352 WP_000354046.1 NZ_CP086678:2979008-2979274 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224352 NZ_CP120211:c2042419-2042168 [Agrobacterium leguminum]
ATGCGTACCGTTATGTTTTCAAAAGCACGCGAATCCCTTGCATCGCTTCTGGATGAAGTGTCGCAGGACCGGGTGACTGT
GGAAATCGTGCGCCGCGACAAGCCGTCCGCCGTTCTTATCGATAAGGACGAGTATGAAGGCTTGATGGAAACCTTGCATC
TCTGCTCGTCGCCGGAGAATTTCGCCAGACTTAGAAAGTCGATGTCCGATGTGGACGCGGGTACTTTCGAAAGCCATGAT
CTTATCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References