Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 1557535..1558032 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LMF34_RS07470 Protein ID WP_001173343.1
Coordinates 1557535..1557708 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LMF34_RS07475 Protein ID WP_000398619.1
Coordinates 1557736..1558032 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS07460 (1554205) 1554205..1555662 + 1458 WP_000856827.1 dipeptide/tripeptide permease DtpC -
LMF34_RS07465 (1555899) 1555899..1557416 + 1518 WP_000003806.1 lysine--tRNA ligase -
LMF34_RS07470 (1557535) 1557535..1557708 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LMF34_RS07475 (1557736) 1557736..1558032 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LMF34_RS07480 (1558261) 1558261..1558533 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LMF34_RS07485 (1558545) 1558545..1558775 - 231 WP_000373291.1 4Fe-4S mono-cluster protein YjdI -
LMF34_RS07490 (1558956) 1558956..1560587 + 1632 WP_001216477.1 sensor histidine kinase -
LMF34_RS07495 (1560584) 1560584..1561303 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 1557535..1566407 8872


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224348 WP_001173343.1 NZ_CP086678:c1557708-1557535 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224348 NZ_CP120211:c523418-523119 [Agrobacterium leguminum]
ATGGCCGTGACGGAATTTAGCCAATTCGATGTGTCGGACTATCTGGATAGCGATGAAGCCATGATCGAATATCTGGCGGT
TGCGATGGAAGATGAAAACCCTGACGTGTTCATTGCCGCCCTTGGCGCCGTCGCCAGAGCTCGCGGAATGTCACATGTTG
CTGATGCTTCGGGTCTGGGTCGCGAAAGCCTCTACAAAGCACTTAAGCCCGGTTCGAAGCTTCGCTATGAAACCGTGCAG
AAAGTCCTGCATGCGCTGGGCTTCCGACTGGCCGTCGAAAAATCATTGAGCGCCGCGTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224348 WP_000398619.1 NZ_CP086678:c1558032-1557736 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224348 NZ_CP120211:c523713-523399 [Agrobacterium leguminum]
ATGAACAGCTTCGAGAAATCGGCAGAGTTCGACGAATGGCTGAAGGCATTGAAAGATCGTGCCGGAAAGGTGCGGATTCT
TCATCGTCTCACCGCGGCCGAGCATGGGAATTTCGGCGATTGTGAGCCGGTGGGTGGAGGCGTTTCAGAAATGCGTATCC
ACTACGGCCCGGGTTATCGGGTCTATTTCACCCGCGTTGGCAAGAGGATTTACTTCCTTCTGATCGGCGGGGACAAGTCT
TCGCAAAAGCGGGACATAAAACGCGCAATCGGGATGGCGCAGACAATAGGAAGGGATGGCCGTGACGGAATTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References