Detailed information of TA system TA224347

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 1458293..1458888
• Replicon: chromosome
• Accession: NZ_CP086678
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: LMF34_RS06960
• Protein ID: WP_000239581.1
• Coordinates: 1458293..1458643 (-)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: LMF34_RS06965
• Protein ID: WP_001223213.1
• Coordinates: 1458637..1458888 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS06940 (1453747)	1453747..1454769	-	1023	WP_001361374.1	ABC transporter permease	-
LMF34_RS06945 (1454783)	1454783..1456285	-	1503	WP_001522399.1	sugar ABC transporter ATP-binding protein	-
LMF34_RS06950 (1456418)	1456418..1457374	-	957	WP_001522398.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
LMF34_RS06955 (1457684)	1457684..1458214	+	531	WP_000055075.1	inorganic diphosphatase	-
LMF34_RS06960 (1458293)	1458293..1458643	-	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
LMF34_RS06965 (1458637)	1458637..1458888	-	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
LMF34_RS06970 (1459100)	1459100..1459441	-	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
LMF34_RS06975 (1459444)	1459444..1463223	-	3780	WP_001522396.1	autotransporter assembly complex protein TamB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T224347 WP_000239581.1 NZ_CP086678:c1458643-1458293 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T224347 NZ_CP120211:475097-475387 [Agrobacterium leguminum]
ATGGGCTTTCGCCTGTCTCTGCTCGCGGAAGAGGACGTCATTCGCATAGTCGAAGAAGGCATCGTCTTATTCGGGCTGGC
GCAGGCGCAAAAATATCACCGGGAACTCTATGAGATATTTGAGCTGATATCTCGCAACCCACGAATGGCACGAGAGCGGC
ATGAGTTGTTACCGCCGCTGCGTATCCATCGGTTCAGGGCACATCTGGTTGTTTATGCCGTGGGAACGGACGATGACGTC
CTGATCGTCCGCGTCCGCCACGCACATGAAGACTGGGCCAACGAGTTGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT224347 WP_001223213.1 NZ_CP086678:c1458888-1458637 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT224347 NZ_CP120211:474843-475094 [Agrobacterium leguminum]
ATGGCGACCATGAACATATCGCTGCCTGCCCCCATGAAAGAATGGGTCGAGGCCCAATCCAGGACAGGTCGTTACTCCAA
TGCGAGTGACTACGTGCGAGACCTGATCCGCAAGGATCAGGAACGAGGCGAAAAAATCGCGGCGATGCAGCGTTTTGTGG
ATGAGGGACTGCAGAGTGGCGTCGGGTATCGCTCGCAGGAGGCCCTGTTCGCCGAGGCTGAAGCCCGCTCGATGAAACGC
AAAATCGGTTGA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7