Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 799521..800139 Replicon chromosome
Accession NZ_CP086678
Organism Escherichia coli strain EFF60

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LMF34_RS03915 Protein ID WP_001291435.1
Coordinates 799921..800139 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LMF34_RS03910 Protein ID WP_000344800.1
Coordinates 799521..799895 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LMF34_RS03900 (794610) 794610..795803 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LMF34_RS03905 (795826) 795826..798975 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LMF34_RS03910 (799521) 799521..799895 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LMF34_RS03915 (799921) 799921..800139 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LMF34_RS03920 (800311) 800311..800862 + 552 WP_001522034.1 maltose O-acetyltransferase -
LMF34_RS03925 (800978) 800978..801448 + 471 WP_001490888.1 YlaC family protein -
LMF34_RS03930 (801612) 801612..803162 + 1551 WP_001577872.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LMF34_RS03935 (803204) 803204..803557 - 354 WP_000878140.1 DUF1428 domain-containing protein -
LMF34_RS03940 (803901) 803901..804128 - 228 WP_024183738.1 DUF2188 domain-containing protein -
LMF34_RS03945 (804346) 804346..804966 - 621 WP_001267012.1 recombinase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224343 WP_001291435.1 NZ_CP086678:799921-800139 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224343 NZ_CP119987:c224056-223904 [Salmonella enterica]
ATGCCACAGCGAACGTTTTTAATGATGTTAATCGTCGTCTGTGTGACGATTCTGTGTTTTGTCTGGATGGTGAGGGATTC
GCTTTGCGGACTCCGGCTCCAGCAGGGAAACACAGTGCTTGTGGCAACGTTAGCCTACGAAGTTAAACGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224343 WP_000344800.1 NZ_CP086678:799521-799895 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224343 NZ_CP119987:224108-224168 [Salmonella enterica]
GAGGCATTACAGTAGAGCCCTTGGTTGATTGATAAAAAAATCTCCAGGGGCTTTCTGCTGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References