Detailed information of TA system TA224342

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 41801..42585
• Replicon: chromosome
• Accession: NZ_CP086678
• Organism: Escherichia coli strain EFF60

Toxin (Protein)

• Gene name: TacT2
• Uniprot ID: V0T0H9
• Locus tag: LMF34_RS00215
• Protein ID: WP_000613626.1
• Coordinates: 42091..42585 (+)
• Length: 165 a.a.

Antitoxin (Protein)

• Gene name: TacA2
• Uniprot ID: L4JCW6
• Locus tag: LMF34_RS00210
• Protein ID: WP_001110447.1
• Coordinates: 41801..42094 (+)
• Length: 98 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LMF34_RS00200 (36951)	36951..37910	-	960	WP_000846343.1	23S rRNA pseudouridine(955/2504/2580) synthase RluC	-
LMF34_RS00205 (38483)	38483..41668	+	3186	WP_001522867.1	ribonuclease E	-
LMF34_RS00210 (41801)	41801..42094	+	294	WP_001110447.1	DUF1778 domain-containing protein	Antitoxin
LMF34_RS00215 (42091)	42091..42585	+	495	WP_000613626.1	GNAT family N-acetyltransferase	Toxin
LMF34_RS00220 (42680)	42680..43633	-	954	WP_001212768.1	flagellar hook-associated protein FlgL	-
LMF34_RS00225 (43645)	43645..45288	-	1644	WP_001522865.1	flagellar hook-associated protein FlgK	-
LMF34_RS00230 (45354)	45354..46295	-	942	WP_001522863.1	flagellar assembly peptidoglycan hydrolase FlgJ	-
LMF34_RS00235 (46295)	46295..47392	-	1098	WP_000589326.1	flagellar basal body P-ring protein FlgI	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (81-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (14-88)

Sequences

Toxin

Protein

• Length: 165 a.a.
• Molecular weight: 18084.00 Da
• Isoelectric Point: 8.2617

>T224342 WP_000613626.1 NZ_CP086678:42091-42585 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Nucleotide

• Length: 495 bp

>T224342 NZ_CP119987:50117-50422 [Salmonella enterica]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTACCGCCTGTTTGTGGATGTGCAGAGCGACATCGTCGATAC
CCCTGGCCGGCGAATGGTGATCCCGCTGGCCAGTGCCCGTCTGCTGTCGGATAAGGTCTCCCGCGAACTTTATCCGGTGG
TGCATATCGGGGATGAGAGCTGGCGTCTGATGACCACGGATATGGCCAGTGTCCCGGCCTCATTCATCGGGGAAGAGGTG
GCCGAGCTCAGCCACAGGGAGAACGACATCAAAAACGCCATTAACCTGATGTTCTGGGGGATCTGA

Antitoxin

Protein

• Length: 98 a.a.
• Molecular weight: 11109.69 Da
• Isoelectric Point: 10.5423

>AT224342 WP_001110447.1 NZ_CP086678:41801-42094 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Nucleotide

• Length: 294 bp

>AT224342 NZ_CP119987:49897-50115 [Salmonella enterica]
ATGAAGCAGCGTATTACTGTCACCATAGACAGTGACAGCTATCAGTTACTCAAGTCCGCAGACGTGAATATCTCCGGCCT
GGTAAATACTGCCATGCAGAAGGAGGCCCGCCGTCTGCGTGCGGAACGCTGGCAGGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAGCGAACGGCTCCTTTGCAGACGAAAACAGGGACTGGTGA

Structures

Toxin

• AlphaFold DB: V0T0H9

Antitoxin

• AlphaFold DB: L4JCW6