Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 17449..18092 | Replicon | plasmid pKPC-2 |
| Accession | NZ_CP086668 | ||
| Organism | Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a) | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | D8L2J9 |
| Locus tag | KFX87_RS29820 | Protein ID | WP_001044770.1 |
| Coordinates | 17676..18092 (+) | Length | 139 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | D5KTK7 |
| Locus tag | KFX87_RS29815 | Protein ID | WP_001261282.1 |
| Coordinates | 17449..17679 (+) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFX87_RS29775 (KFX87_029780) | 12647..13120 | + | 474 | WP_004152341.1 | YkgJ family cysteine cluster protein | - |
| KFX87_RS29780 (KFX87_029785) | 13212..13442 | + | 231 | WP_011977773.1 | hypothetical protein | - |
| KFX87_RS29785 (KFX87_029790) | 14334..15116 | - | 783 | WP_004152340.1 | site-specific integrase | - |
| KFX87_RS29790 (KFX87_029795) | 15116..15448 | - | 333 | WP_004152339.1 | hypothetical protein | - |
| KFX87_RS29795 (KFX87_029800) | 15455..15853 | - | 399 | WP_004171440.1 | hypothetical protein | - |
| KFX87_RS29800 (KFX87_029805) | 15879..16208 | - | 330 | WP_004152337.1 | hypothetical protein | - |
| KFX87_RS29805 (KFX87_029810) | 16236..16544 | - | 309 | WP_004152336.1 | hypothetical protein | - |
| KFX87_RS29810 (KFX87_029815) | 17031..17492 | - | 462 | WP_072093212.1 | hypothetical protein | - |
| KFX87_RS29815 (KFX87_029820) | 17449..17679 | + | 231 | WP_001261282.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| KFX87_RS29820 (KFX87_029825) | 17676..18092 | + | 417 | WP_001044770.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KFX87_RS29825 (KFX87_029830) | 18166..18855 | + | 690 | Protein_19 | AAA family ATPase | - |
| KFX87_RS29830 (KFX87_029835) | 18910..19607 | + | 698 | WP_223174630.1 | IS1 family transposase | - |
| KFX87_RS29835 (KFX87_029840) | 19671..20081 | + | 411 | WP_004152401.1 | hypothetical protein | - |
| KFX87_RS29840 (KFX87_029845) | 20738..21097 | - | 360 | WP_117046906.1 | DUF305 domain-containing protein | - |
| KFX87_RS29845 (KFX87_029850) | 21269..21499 | - | 231 | Protein_23 | IS5/IS1182 family transposase | - |
| KFX87_RS29850 (KFX87_029855) | 21601..22785 | + | 1185 | WP_004152398.1 | ISAs1-like element ISKpn31 family transposase | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaKPC-2 | - | 1..102252 | 102252 | |
| - | flank | IS/Tn | - | - | 11259..12527 | 1268 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 139 a.a. Molecular weight: 15106.52 Da Isoelectric Point: 7.1084
>T224341 WP_001044770.1 NZ_CP086668:17676-18092 [Klebsiella pneumoniae]
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
VNKTYMLDTCICSFIMREQPEAVLKRLEQAVLRGQRIVVSAITYSEMRFGATGPKASPRHVELVDAFCARLDAILPWDRA
AVDATTEIKVALRMAGTPIGPNDTAIAGHAIAAGAVLVTNNTREFERVPGLVLEDWVR
Download Length: 417 bp
>T224341 NZ_CP119987:c49046-48630 [Salmonella enterica]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA
Antitoxin
Download Length: 77 a.a. Molecular weight: 8756.83 Da Isoelectric Point: 4.4692
>AT224341 WP_001261282.1 NZ_CP086668:17449-17679 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
MRTVSIFKNGNNRAIRLPRDLDFEGVSELEIVREGDSIILRPVRPTWGSFAQFEKADPDFMAEREDVVSDEGRFNL
Download Length: 231 bp
>AT224341 NZ_CP119987:c49273-49043 [Salmonella enterica]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GYM2 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A0H3GZG3 |