Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 56068..56804 Replicon plasmid pIncFIB
Accession NZ_CP086667
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name tacT Uniprot ID L7SZ15
Locus tag KFX87_RS29105 Protein ID WP_003026803.1
Coordinates 56322..56804 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID L7SZ29
Locus tag KFX87_RS29100 Protein ID WP_003026799.1
Coordinates 56068..56334 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS29055 (KFX87_029050) 51342..51692 - 351 WP_004152101.1 As(III)-sensing metalloregulatory transcriptional repressor ArsR -
KFX87_RS29060 (KFX87_029055) 52050..52319 + 270 WP_004152102.1 hypothetical protein -
KFX87_RS29065 (KFX87_029060) 52307..52882 + 576 WP_004152103.1 hypothetical protein -
KFX87_RS29070 (KFX87_029065) 52913..53407 + 495 WP_004152104.1 DNA-binding protein -
KFX87_RS29075 (KFX87_029070) 53451..53819 + 369 WP_004152105.1 hypothetical protein -
KFX87_RS29080 (KFX87_029075) 53853..54056 + 204 WP_004152106.1 HHA domain-containing protein -
KFX87_RS29085 (KFX87_029080) 54105..54362 + 258 WP_004152107.1 hypothetical protein -
KFX87_RS29090 (KFX87_029085) 54658..55638 - 981 WP_000019473.1 IS5-like element ISKpn26 family transposase -
KFX87_RS29095 (KFX87_029090) 55707..55892 + 186 Protein_56 hypothetical protein -
KFX87_RS29100 (KFX87_029095) 56068..56334 + 267 WP_003026799.1 DUF1778 domain-containing protein Antitoxin
KFX87_RS29105 (KFX87_029100) 56322..56804 + 483 WP_003026803.1 GNAT family N-acetyltransferase Toxin
KFX87_RS29110 (KFX87_029105) 57016..58362 + 1347 WP_020314316.1 ISNCY family transposase -
KFX87_RS30435 58523..58654 + 132 WP_004218042.1 hypothetical protein -
KFX87_RS29115 (KFX87_029110) 58863..60028 - 1166 Protein_61 IS3 family transposase -
KFX87_RS29120 (KFX87_029115) 60205..61167 - 963 WP_004152113.1 zinc metalloprotease HtpX -
KFX87_RS29125 (KFX87_029120) 61154..61642 - 489 WP_004152114.1 phosphate-starvation-inducible PsiE family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid - - 1..171935 171935
- inside IScluster/Tn - - 54658..59890 5232


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(45-149)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17353.97 Da        Isoelectric Point: 9.5822

>T224340 WP_003026803.1 NZ_CP086667:56322-56804 [Klebsiella pneumoniae]
VGRITTPEPLSSSHQLAEFVSGETVLDEWLKQRGLKNQALGAARTFVVCKTGTKQVAGFYSLATGSVNHTEATGSLRRNM
PDPIPVIILARLAVDVSLHGKGVGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKGFYAHHGFKASQTHERTLFLKLP

Download         Length: 483 bp

>T224340 NZ_CP119987:28886-29368 [Salmonella enterica]
ATGGAAATAAGTGTTACTGCACCGGAGTTGCTAAACGAGGAACACTATCTTCAACAGTTTGATTGCGGGAATGATGTGCT
CAGTGACTGGCTTCGCCGCAGAGCGATGAAGAACCAATACCTCAATGCCTCCCGTACATTTGTTATCTGCCCTGAAGGCA
CAAAGCGAGTAGTGGGATATTACTCTATTGCGACGGGGTCTGTAAGTCATGCCAGTTTAGGTCGCAGTTTACGCCAGAAT
ATGCCCGATCCTGTTCCGGTTGTTTTGCTGGGACGTCTTGCTGTTGATGAGTGTACTCAAGGGCATAGTTTTGGAAAATG
GCTGCTTAATGATGCTGTAACACGGGTATCTAACCTGGCAGACCAGGTGGGTATTAAAGCGATCATGGTTCATGCTATTG
ATGAACAGGCGAAAACATTCTATGAATACTTTGGCTTTGTTCAGTCTCCCATTGCGCCAAATACATTGTTCTACAAGATT
TAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10147.59 Da        Isoelectric Point: 4.5256

>AT224340 WP_003026799.1 NZ_CP086667:56068-56334 [Klebsiella pneumoniae]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACKAAENVILDRRVFNFNDEQYAEFIDMLDAPVEDEPAINKLL
ARKPQWDV

Download         Length: 267 bp

>AT224340 NZ_CP119987:28611-28895 [Salmonella enterica]
ATGCAAACTACAATCCGCAAATCTGTGCGTAATAAACAAATAAATATACGGGCTACTGATGAGGAACGTGCTGTGATTGA
TTATGCAGCGAGCCTGGTCAGTAAAAACCGTACCGACTTCATCATAGAAAAGGCTGTGAGCGAGGCCCAGAATATCATTC
TGGATCAACGCGTTTTTGTTCTGGATGATGCCCGCTATCAGGCTTTTATTAAACAACTTGAAGCTCCTGTACAGAATACT
GAAGGTCGTCAGCGCCTGATGGATGTGAAACCTGAATGGAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0J2GNW6

Antitoxin

Source ID Structure
AlphaFold DB A0A1Q8YL66

References