Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vagCD/VapC-VagC |
| Location | 278056..278726 | Replicon | plasmid pOXA-48 |
| Accession | NZ_CP086665 | ||
| Organism | Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a) | ||
Toxin (Protein)
| Gene name | vagD | Uniprot ID | Q6U619 |
| Locus tag | KFX87_RS27575 | Protein ID | WP_004213072.1 |
| Coordinates | 278056..278499 (-) | Length | 148 a.a. |
Antitoxin (Protein)
| Gene name | vagC | Uniprot ID | Q6U620 |
| Locus tag | KFX87_RS27580 | Protein ID | WP_004213073.1 |
| Coordinates | 278496..278726 (-) | Length | 77 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| KFX87_RS27545 (KFX87_027540) | 273892..274295 | + | 404 | Protein_307 | GAF domain-containing protein | - |
| KFX87_RS27550 (KFX87_027545) | 274813..275168 | - | 356 | Protein_308 | mucoid phenotype regulator RmpA2 | - |
| KFX87_RS27555 (KFX87_027550) | 275398..276594 | + | 1197 | WP_039023277.1 | IS110-like element ISEc20 family transposase | - |
| KFX87_RS27560 (KFX87_027555) | 276628..276912 | - | 285 | Protein_310 | mucoid phenotype regulator RmpA2 | - |
| KFX87_RS27565 (KFX87_027560) | 277329..277633 | + | 305 | Protein_311 | transposase | - |
| KFX87_RS27570 (KFX87_027565) | 277656..277907 | - | 252 | WP_186987481.1 | hypothetical protein | - |
| KFX87_RS27575 (KFX87_027570) | 278056..278499 | - | 444 | WP_004213072.1 | type II toxin-antitoxin system VapC family toxin | Toxin |
| KFX87_RS27580 (KFX87_027575) | 278496..278726 | - | 231 | WP_004213073.1 | type II toxin-antitoxin system VapB family antitoxin | Antitoxin |
| KFX87_RS27585 (KFX87_027580) | 279334..280467 | + | 1134 | WP_004213075.1 | DUF3800 domain-containing protein | - |
| KFX87_RS27590 (KFX87_027585) | 280483..280776 | + | 294 | WP_004213076.1 | hypothetical protein | - |
| KFX87_RS27595 (KFX87_027590) | 280766..280972 | - | 207 | WP_004213077.1 | hypothetical protein | - |
| KFX87_RS27600 (KFX87_027595) | 281324..281614 | + | 291 | WP_004213078.1 | hypothetical protein | - |
| KFX87_RS27605 (KFX87_027600) | 281604..282503 | + | 900 | WP_004225022.1 | nucleotide-binding protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1 | iucA / iucB / iucC / iucD / iutA / rmpA2 | 1..323074 | 323074 | |
| - | flank | IS/Tn | - | - | 275398..276594 | 1196 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 148 a.a. Molecular weight: 16006.74 Da Isoelectric Point: 9.3642
>T224338 WP_004213072.1 NZ_CP086665:c278499-278056 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS
Download Length: 444 bp
>T224338 NZ_CP119986:4693996-4694307 [Salmonella enterica]
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
ATGCAATTTATAGAAACGGAACTCTTTACTGAAGATGTTAAAAAACTGCTCGATGATGATGAATACCATAAGCTTCAGGT
TTTTATGGCTCAGCATCCAGATTGTGGTGATGTCATTCAGGAAACGGGCGGCCTGAGAAAAATGCGCTGGGGAGCGCGAG
GCAAAGGAAAGCGTAGTGGCGTGCGAATTATCTATTTTCACCGTAGTCAACGGTATGAGATTCGCTTGCTTCTGATTTAT
CAAAAAGGCATTAAAGATGATCTCACGCCGCAGGAAAAAGCGGTGCTTCGTATGCTGAATGAGAGGTGGTAG
Antitoxin
Download Length: 77 a.a. Molecular weight: 8677.73 Da Isoelectric Point: 4.4563
>AT224338 WP_004213073.1 NZ_CP086665:c278726-278496 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP
Download Length: 231 bp
>AT224338 NZ_CP119986:4694308-4694598 [Salmonella enterica]
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
ATGGATAAAGTGTTATTTGAGCGATTAACTCAAAGTATGTCTCAAATGAATGAAATCATTGAAGGGACCCGTGAACCTTC
TCGTACCTTTCATATTGATGCAATGAAGATTAAAGAAATACGGCAGGCATCTGGGTTGTCGCAATCTAAGTTTGCAGAGC
TGATTTCGGTCAACGTGGATACGCTGCGCAACTGGGAGCAAGGAAGACGTTCACCGACAGGGCCAGCCAAAGCGTTACTT
CGCGCCATTGCCAACGACCCGAGAAACGTTATACAAGCATTGCGTTATTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|