Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 145307..146217 Replicon plasmid pOXA-48
Accession NZ_CP086665
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name - Uniprot ID A0A663AYG0
Locus tag KFX87_RS26865 Protein ID WP_004026354.1
Coordinates 145747..146217 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A6P1V3Q9
Locus tag KFX87_RS26860 Protein ID WP_004026357.1
Coordinates 145307..145750 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS26835 (KFX87_026830) 140463..140678 - 216 Protein_163 IS200/IS605 family transposase -
KFX87_RS26840 (KFX87_026835) 140680..141249 + 570 Protein_164 transposase -
KFX87_RS26845 (KFX87_026840) 141617..143104 - 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
KFX87_RS26850 (KFX87_026845) 143514..143936 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
KFX87_RS26855 (KFX87_026850) 143936..145207 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
KFX87_RS26860 (KFX87_026855) 145307..145750 + 444 WP_004026357.1 DUF2384 domain-containing protein Antitoxin
KFX87_RS26865 (KFX87_026860) 145747..146217 + 471 WP_004026354.1 RES family NAD+ phosphorylase Toxin
KFX87_RS26870 (KFX87_026865) 146326..146586 - 261 WP_004026352.1 hypothetical protein -
KFX87_RS26880 (KFX87_026875) 147613..148272 + 660 WP_000412211.1 type A-1 chloramphenicol O-acetyltransferase -
KFX87_RS26885 (KFX87_026880) 148473..148850 - 378 WP_000656305.1 GNAT family N-acetyltransferase -
KFX87_RS26890 (KFX87_026885) 148917..149750 - 834 Protein_174 Tn3 family transposase -
KFX87_RS26895 (KFX87_026890) 149815..150519 + 705 WP_001067855.1 IS6-like element IS26 family transposase -
KFX87_RS26900 (KFX87_026895) 150573..151013 - 441 Protein_176 CatB-related O-acetyltransferase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Conjugative plasmid blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1 iucA / iucB / iucC / iucD / iutA / rmpA2 1..323074 323074
- flank IS/Tn - - 140896..141249 353


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17515.79 Da        Isoelectric Point: 4.6155

>T224337 WP_004026354.1 NZ_CP086665:145747-146217 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T224337 NZ_CP119986:c4692763-4692452 [Salmonella enterica]
GTGCATGTTATCAGCCGAAAACCGTTCAATGAAGCGATGCTCATGTATCCAAACCATGAGCTTGCTCTCACTGAGTTGCT
GAATGTTCTGGAGAAAAAAACATTCACTCAGCCTGAAGAGATGAAGCGGTATATTCCTTCACTGGATAATTTTAAGTACA
GAGATAAATGGTGGGTGATTGATGTTTCTGGTAACAGCCTGAGACTCATTTCCTACATAGATTTCAGGTTACATAAGATA
TTTGTGAAACATATCGTTTCCCATGCCGAATATGACAAACTGACCGCATACTATCGGGGTAATAAAGAATGA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16480.80 Da        Isoelectric Point: 10.2498

>AT224337 WP_004026357.1 NZ_CP086665:145307-145750 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDKEAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT224337 NZ_CP119986:c4692455-4692009 [Salmonella enterica]
ATGAGAACTCATCGTCAGATGGATGCAACCAGCGCAAAAAAGATCGTTGATACCTTCAGCGATGCGGTAAAAACCGTCCC
ACTGATGGGGGAAGACCGAAATGACAATGAGTATCGCAGGGCACTAGCGCTAGTGGAGTTTCTGGTCGACCACGACGATC
TTGAAAACCCACTATTTGAATTGCTCTGTGCCCGAATCAGTGAATACGAAAAACATGCGCCGGAATTCAAAGCACTCAAC
CAACACCTGGAGAAAACGCCCCCTGGCGTTTCAGTATTGCGAACGCTCATGGATCAATACGGTCTCAAAGCAGCAGATCT
TGCCAACGAACTTGGTTCTAAATCGAACGTCAGCAACATCTTAAATGGCCGCAGAGCACTAACGGTTAATCATATTAAAG
CGCTTACACAACGCTTCAAACTACCAGCAGATGCCTTCATCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A663AYG0

Antitoxin

Source ID Structure
AlphaFold DB A0A6P1V3Q9

References