Detailed information of TA system TA224336

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-DnaT
• Location: 45929..46451
• Replicon: plasmid pOXA-48
• Accession: NZ_CP086665
• Organism: Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: A0A2J4R0S6
• Locus tag: KFX87_RS26335
• Protein ID: WP_004181778.1
• Coordinates: 46167..46451 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A5Q9LM53
• Locus tag: KFX87_RS26330
• Protein ID: WP_025368601.1
• Coordinates: 45929..46177 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFX87_RS26310 (KFX87_026305)	41139..41975	-	837	WP_223686625.1	hypothetical protein	-
KFX87_RS26315 (KFX87_026310)	42022..42375	-	354	WP_004181774.1	hypothetical protein	-
KFX87_RS26320 (KFX87_026315)	42520..43506	-	987	WP_025368599.1	hypothetical protein	-
KFX87_RS26325 (KFX87_026320)	43840..45639	-	1800	WP_088678991.1	ATP-dependent helicase	-
KFX87_RS26330 (KFX87_026325)	45929..46177	+	249	WP_025368601.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
KFX87_RS26335 (KFX87_026330)	46167..46451	+	285	WP_004181778.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFX87_RS26340 (KFX87_026335)	46681..47907	+	1227	WP_025368602.1	RNA-guided endonuclease TnpB family protein	-
KFX87_RS26345 (KFX87_026340)	48193..48618	-	426	WP_040120489.1	IS200/IS605 family transposase	-
KFX87_RS26350 (KFX87_026345)	48655..49881	+	1227	WP_025368604.1	RNA-guided endonuclease TnpB family protein	-
KFX87_RS26355 (KFX87_026350)	49947..51073	-	1127	Protein_66	conjugal transfer protein TraG N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaOXA-48 / catA1 / blaOXA-1 / aac(6')-Ib-cr / blaTEM-1B / qnrS1 / blaCTX-M-15 / ant(2'')-Ia / ant(3'')-Ia / qacE / sul1	iucA / iucB / iucC / iucD / iutA / rmpA2	1..323074	323074

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10970.69 Da
• Isoelectric Point: 10.6516

>T224336 WP_004181778.1 NZ_CP086665:46167-46451 [Klebsiella pneumoniae]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Nucleotide

• Length: 285 bp

>T224336 NZ_CP119986:c4298117-4297632 [Salmonella enterica]
GTGGGACGTGTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTAGCTGAGTTCGTCAGCGGTGAAGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCTCTCGGAGCGGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGTAGCGTCAACCATACAGAAGCGACAGGCAACCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAATCATCACAAACTCAGCAGCGAACATTGTTCCTTAGGCTCCCT
CAATAG

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 8906.12 Da
• Isoelectric Point: 3.8206

>AT224336 WP_025368601.1 NZ_CP086665:45929-46177 [Klebsiella pneumoniae]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGHIADERIGEAVIEVSLD
DL

Nucleotide

• Length: 249 bp

>AT224336 NZ_CP119986:c4298392-4298105 [Salmonella enterica]
ATGCTATACAAAGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAACGGCCTGCCAGGCAGCCGAGAAAGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGAGGAGTTCATCAATCTGCTTGATGCACCGGTCGCAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTGTAA

Structures

Toxin

• AlphaFold DB: A0A2J4R0S6

Antitoxin

• AlphaFold DB: A0A5Q9LM53