Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5273329..5273954 Replicon chromosome
Accession NZ_CP086664
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag KFX87_RS25660 Protein ID WP_002882817.1
Coordinates 5273329..5273712 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag KFX87_RS25665 Protein ID WP_004150355.1
Coordinates 5273712..5273954 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS25645 (KFX87_025640) 5270695..5271597 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
KFX87_RS25650 (KFX87_025645) 5271594..5272229 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
KFX87_RS25655 (KFX87_025650) 5272226..5273155 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
KFX87_RS25660 (KFX87_025655) 5273329..5273712 - 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
KFX87_RS25665 (KFX87_025660) 5273712..5273954 - 243 WP_004150355.1 CopG family transcriptional regulator Antitoxin
KFX87_RS25670 (KFX87_025665) 5274159..5275076 + 918 WP_004187175.1 alpha/beta hydrolase -
KFX87_RS25675 (KFX87_025670) 5275090..5276031 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
KFX87_RS25680 (KFX87_025675) 5276076..5276513 - 438 WP_002882809.1 D-aminoacyl-tRNA deacylase -
KFX87_RS25685 (KFX87_025680) 5276510..5277370 - 861 WP_019725422.1 virulence factor BrkB family protein -
KFX87_RS25690 (KFX87_025685) 5277364..5277963 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T224335 WP_002882817.1 NZ_CP086664:c5273712-5273329 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T224335 NZ_CP119986:c4185581-4185213 [Salmonella enterica]
ATGACCCTACAACTTATCTCAGCGGAAGAGATAATTCAGTTTCACGACAGGCTTCTCCGCGTTACGCCTGGTGTAACAGG
CATGCCTGATCCTGGCCGCGCGGAAGCGCTAATGTACCGGGTACTCAATCAAATCGAATATGAAGGGGTGACCGACGTGT
GGCTGCTGGCGGCAATGCATTTGCTCGCTATATCCCGTTGGCATATCTTCAATGATGGTAACAAACGCACCGCCTTATTT
ATTACGCTGCTGTTTTTAAAGCGTAACGGGATCTCACTCGCTGCGAATCCGGATTTTGTCGATATGACAGTCGATGCGGC
GGCAGGGCGGCTTACGCTGGAGCAAATTGCCGTTCGCTTACGCGCCTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT224335 WP_004150355.1 NZ_CP086664:c5273954-5273712 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT224335 NZ_CP119986:c4185799-4185578 [Salmonella enterica]
ATGCGTACGGTTAACTATAGCGAAGCGCGGCAAAATCTGGCCGAAGTCCTGGAAAGCGCGGTGACGGCGGGCCCTGTCAC
CATCACGCGTCGTGGGCATAAGTCCGCAGTGATCATCAGCGCCGAGGAGTTTGAGCGTTATCAGACGGCGCGAATGGATG
ATGAGTTTGCTGCCATTATGGCGGTTCATGGCAATGAGCTCAGGGATCTGGCGGATAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References