Detailed information of TA system TA224333

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-RelB
• Location: 4792008..4792524
• Replicon: chromosome
• Accession: NZ_CP086664
• Organism: Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)

• Gene name: relE
• Uniprot ID: R4YAY3
• Locus tag: KFX87_RS23375
• Protein ID: WP_004178374.1
• Coordinates: 4792008..4792292 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: R4Y888
• Locus tag: KFX87_RS23380
• Protein ID: WP_002886901.1
• Coordinates: 4792282..4792524 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KFX87_RS23350 (KFX87_023345)	4787425..4787688	-	264	WP_025987940.1	PTS sugar transporter subunit IIB	-
KFX87_RS23355 (KFX87_023350)	4787818..4787991	+	174	WP_019725541.1	hypothetical protein	-
KFX87_RS23360 (KFX87_023355)	4787994..4788737	+	744	WP_002886905.1	MurR/RpiR family transcriptional regulator	-
KFX87_RS23365 (KFX87_023360)	4789094..4791232	+	2139	WP_019725540.1	anaerobic ribonucleoside-triphosphate reductase	-
KFX87_RS23370 (KFX87_023365)	4791540..4792004	+	465	WP_004178375.1	anaerobic ribonucleoside-triphosphate reductase-activating protein	-
KFX87_RS23375 (KFX87_023370)	4792008..4792292	-	285	WP_004178374.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KFX87_RS23380 (KFX87_023375)	4792282..4792524	-	243	WP_002886901.1	type II toxin-antitoxin system RelB/DinJ family antitoxin	Antitoxin
KFX87_RS23385 (KFX87_023380)	4792602..4794512	-	1911	WP_019725539.1	PRD domain-containing protein	-
KFX87_RS23390 (KFX87_023385)	4794535..4795689	-	1155	WP_004178372.1	lactonase family protein	-
KFX87_RS23395 (KFX87_023390)	4795756..4796496	-	741	WP_002886899.1	KDGP aldolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

• RelB antitoxin/Antitoxin DinJ (1-80)

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 11155.97 Da
• Isoelectric Point: 10.3787

>T224333 WP_004178374.1 NZ_CP086664:c4792292-4792008 [Klebsiella pneumoniae]
MTYELEFDPRAWREWQKPGETVKKQFKNKLQQIVQNPRIESTRLSDLPDCYKIKLKASGYRLVYQVRDSVVVVYVIAIGK
REKAAVYHQANKRL

Nucleotide

• Length: 285 bp

>T224333 NZ_CP119986:3488105-3488632 [Salmonella enterica]
ATGATGTTTACAGACTGGCATGAGGCCGCGATAGGGAAAACCCACAATCGAATGAATTTTGATTGTGGGGATGCCGATCT
GAACCAATTCCTACAACGCCATGCACTACAAAATCATGAGAAAGGGACAACGAAAACCTATGTTGCGCTTGATAATTCGG
ATGTTACACGTATCCACGGCTTTTACTCAGTCAGTCCTGCATCACTGATATATGCACAGGTTCCCGGCGCAATCAGCAAA
GGATTAGGGAGATATGATGTGCCAGTGTTTCGTCTGGGTCGTTTAGCCGTAGACAAATCTATGCAGGGGCAAGGGCTGGG
AGCACAACTTTTGTTATCTGCCGGAAAGCGTTGCATACAGGCGGCTTTGCAGGTCGGCGGCGTAGCCCTGCTTATTGATG
CCAAAAATAAACAGGTCTGCGACTGGTACAAAGGATTTGGCGCAGTACCATTAAACAATCAACCCCTTTCCTTGTTGCTG
TCGTTTAAAACGCTTTATGCTGCTTTATCTGCATCTGGTAGGTTATGA

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 9045.55 Da
• Isoelectric Point: 6.3340

>AT224333 WP_002886901.1 NZ_CP086664:c4792524-4792282 [Klebsiella pneumoniae]
MATLNVRLDDKLKKEAYAVLEKLDMTPTEAVRLLFQYIAENGRMPVKSMTISDGEAALLRTVRERLENPRPGVKVSLDDL

Nucleotide

• Length: 243 bp

>AT224333 NZ_CP119986:3487818-3488108 [Salmonella enterica]
ATGAAAACCATGCCTCAGATAGCTATAGAATCTAACGAAAGACTGTCGTTACGTGTATCGACGGATGCCAAAAAGTTGAT
AGTCCGCGCTGCGGCAATCCAGCAAACCAACCTGACGGATTTTGTGGTCAGTAACGTACTACCTGTCGCACAGAAGATTG
TTGATGCCGCCGAACGGGTATATCTGACGGAGCGTGATACTCAAATGATCATGGAAATTTTAGATAACCCGCCAGCGCCG
AATGAAAAGCTTCTTGCCGCTGCTTTCGCCCTGCCGGACATGAAGAAATGA

Structures

Toxin

• AlphaFold DB: A6THG1

Antitoxin

• AlphaFold DB: A0A0H3GLP0