Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4667996..4668806 Replicon chromosome
Accession NZ_CP086664
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name KacT Uniprot ID A0A0H3GMP0
Locus tag KFX87_RS22840 Protein ID WP_002887280.1
Coordinates 4667996..4668529 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag KFX87_RS22845 Protein ID WP_002887278.1
Coordinates 4668540..4668806 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS22835 (KFX87_022830) 4666827..4667948 + 1122 WP_009309849.1 cupin domain-containing protein -
KFX87_RS22840 (KFX87_022835) 4667996..4668529 - 534 WP_002887280.1 type II toxin-antitoxin system toxin KacT Toxin
KFX87_RS22845 (KFX87_022840) 4668540..4668806 - 267 WP_002887278.1 type II toxin-antitoxin system antitoxin KacA Antitoxin
KFX87_RS22850 (KFX87_022845) 4668909..4670342 - 1434 WP_019724905.1 Cu(+)/Ag(+) sensor histidine kinase -
KFX87_RS22855 (KFX87_022850) 4670332..4671015 - 684 WP_004214143.1 copper response regulator transcription factor CusR -
KFX87_RS22860 (KFX87_022855) 4671187..4672569 + 1383 WP_019724904.1 efflux transporter outer membrane subunit -
KFX87_RS22865 (KFX87_022860) 4672587..4672931 + 345 WP_025987660.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19838.70 Da        Isoelectric Point: 5.2614

>T224331 WP_002887280.1 NZ_CP086664:c4668529-4667996 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHVMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDENSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T224331 NZ_CP119986:c2142633-2142349 [Salmonella enterica]
ATGACTTATAAGCTGGCATTTAACGAATCAGCACTAAAAGAGTGGAAAAAACTGGGTCATACCATACAGGAACAGTTTAA
AAAAAAGCTAAGGGAACGGCTTGAAAACCCTCGGGTTCCGGCTTCTCAATTACATGGAAGAAAGGATCAGTACAAAATTA
AGCTGCGTGGTGCAGGATACAGACTGGTGTACAGCGTCGAAGATGAAATTATCACGGTAACTGTCATTGGTGTTGGAAAG
CGTGAAAATGATGCCGTCTACAAAATGACACGACACAGAAGCTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT224331 WP_002887278.1 NZ_CP086664:c4668806-4668540 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT224331 NZ_CP119986:c2142871-2142623 [Salmonella enterica]
ATGGCATTTCAAATTTTAACGACTACTGCAGCAAGCATTACAGAACTGAAACGTGATCCAATGGGAACTTTCAATGCCGG
AGAGGGCGCTCCAGTTGCCATTCTTAACCGTAATGAACCGGCTTTCTACTGTGTGCCTCCGGCACTTTATGCTCATCTTA
TGGATATTCTTGAGGATGAAGAACTCGGTCGTATTATCGATGAGCGTGCGAATGAACGCGTCATTGAGGTCAACATTGAT
GACTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5ZGN
PDB 5XUN

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References