Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4052638..4053257 Replicon chromosome
Accession NZ_CP086664
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name Hha Uniprot ID R8WYV2
Locus tag KFX87_RS19870 Protein ID WP_002892050.1
Coordinates 4053039..4053257 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID J2DPF6
Locus tag KFX87_RS19865 Protein ID WP_002892066.1
Coordinates 4052638..4053012 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS19855 (KFX87_019850) 4047790..4048983 + 1194 WP_004177236.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
KFX87_RS19860 (KFX87_019855) 4049006..4052152 + 3147 WP_002892069.1 multidrug efflux RND transporter permease subunit AcrB -
KFX87_RS19865 (KFX87_019860) 4052638..4053012 + 375 WP_002892066.1 Hha toxicity modulator TomB Antitoxin
KFX87_RS19870 (KFX87_019865) 4053039..4053257 + 219 WP_002892050.1 HHA domain-containing protein Toxin
KFX87_RS19875 (KFX87_019870) 4053420..4053986 + 567 WP_002892030.1 maltose O-acetyltransferase -
KFX87_RS19880 (KFX87_019875) 4053958..4054098 - 141 WP_004147370.1 hypothetical protein -
KFX87_RS19885 (KFX87_019880) 4054119..4054589 + 471 WP_002892026.1 YlaC family protein -
KFX87_RS19890 (KFX87_019885) 4054564..4056015 - 1452 WP_064145206.1 PLP-dependent aminotransferase family protein -
KFX87_RS19895 (KFX87_019890) 4056116..4056814 + 699 WP_004177238.1 GNAT family protein -
KFX87_RS19900 (KFX87_019895) 4056811..4056951 - 141 WP_002892018.1 type B 50S ribosomal protein L36 -
KFX87_RS19905 (KFX87_019900) 4056951..4057214 - 264 WP_002892011.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224330 WP_002892050.1 NZ_CP086664:4053039-4053257 [Klebsiella pneumoniae]
MSDKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T224330 NZ_CP119986:c1109959-1109741 [Salmonella enterica]
ATGTCTGATAAACCATTAACTAAAACTGATTATTTGATGCGTTTACGGCGCTGTCAGACAATTGACACTCTGGAGCGCGT
CATTGAGAAAAATAAATATGAATTGTCTGACAATGAGCTGGCTGTATTTTACTCAGCTGCGGATCACCGTCTTGCAGAAT
TGACGATGAACAAACTCTACGACAAGATCCCCTCTTCAGTATGGAAATTCATTCGTTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14368.06 Da        Isoelectric Point: 4.8989

>AT224330 WP_002892066.1 NZ_CP086664:4052638-4053012 [Klebsiella pneumoniae]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIATFALNYKIKYAEDNKLIAQVDEYL
DDTFTLFSNYGINSTDLQKWKKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT224330 NZ_CP119986:c1110361-1109987 [Salmonella enterica]
ATGGACGAATACTCACCCAAAAGACATGATATAGCACAGCTTAAATTTCTTTGCGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAATGACCCGACCTCAGCAGTCAACCTGCAGCTCAATGAGCTGATAGAGC
ATATTGCAACTTTCGCGCTTAATTATAAAATTAAGTACAATGAAGACAATAAATTGATTGCGCAGATAGATGAATACCTG
GACGACACCTTTATGTTGTTCAGCAGCTATGGTATTAATACGCAGGATTTGCAAAAATGGCGTAAATCGGGCAACCGCCT
GTTCCGTTGTTTCGTCAACGCCACCAGGGCCAATCCTGTTAGTTTGTCTTGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2P8K6F2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GJ93

References