Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 3067929..3068583 Replicon chromosome
Accession NC_009800
Organism Escherichia coli HS

Toxin (Protein)


Gene name cptA Uniprot ID S1EEB2
Locus tag ECHS_RS15080 Protein ID WP_000244777.1
Coordinates 3067929..3068336 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag ECHS_RS15085 Protein ID WP_000354046.1
Coordinates 3068317..3068583 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
ECHS_RS15060 (EcHS_A3051) 3063886..3065619 - 1734 WP_000813207.1 single-stranded-DNA-specific exonuclease RecJ -
ECHS_RS15065 (EcHS_A3052) 3065625..3066335 - 711 WP_000715216.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
ECHS_RS15070 (EcHS_A3053) 3066360..3067256 - 897 WP_000806639.1 site-specific tyrosine recombinase XerD -
ECHS_RS15075 (EcHS_A3054) 3067368..3067889 + 522 WP_001055874.1 flavodoxin FldB -
ECHS_RS15080 (EcHS_A3055) 3067929..3068336 - 408 WP_000244777.1 toxin CptA Toxin
ECHS_RS15085 3068317..3068583 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
ECHS_RS15090 (EcHS_A3057) 3068826..3069806 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
ECHS_RS15095 (EcHS_A3058) 3070002..3070661 - 660 WP_000250274.1 hemolysin III family protein -
ECHS_RS15100 (EcHS_A3059) 3070825..3071136 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
ECHS_RS15105 (EcHS_A3060) 3071181..3072614 + 1434 WP_001338826.1 6-phospho-beta-glucosidase BglA -
ECHS_RS15110 (EcHS_A3061) 3072671..3073414 - 744 WP_000951948.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16031.96 Da        Isoelectric Point: 11.5202

>T22433 WP_000244777.1 NC_009800:c3068336-3067929 [Escherichia coli HS]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T22433 NC_009800:c3068336-3067929 [Escherichia coli HS]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCACAGTGGCTTTCCTTGCTGATTCATGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCACTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGGCGTATTAATGCTCGCCAGGGGGAAATTCGCTTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCACCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGATGGCGGTAAACGGCA
ACATTTATGGCTGGCAGCCGACAGCATGGACGAAGCTGAATGGCGGGATTTACGGCGGATTTTGTTGCAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT22433 WP_000354046.1 NC_009800:c3068583-3068317 [Escherichia coli HS]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT22433 NC_009800:c3068583-3068317 [Escherichia coli HS]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9LFV7

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References