Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hipBA/HipA-HipB
Location 2674622..2676210 Replicon chromosome
Accession NZ_CP086664
Organism Klebsiella pneumoniae strain SCPM-O-B-8912 (11PKP/19a)

Toxin (Protein)


Gene name hipA Uniprot ID W8UI36
Locus tag KFX87_RS13020 Protein ID WP_002904792.1
Coordinates 2674622..2675944 (-) Length 441 a.a.

Antitoxin (Protein)


Gene name hipB Uniprot ID A0A0H3GXD8
Locus tag KFX87_RS13025 Protein ID WP_002904790.1
Coordinates 2675944..2676210 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
KFX87_RS13005 (KFX87_012995) 2669678..2672179 + 2502 WP_064145444.1 fimbria/pilus outer membrane usher protein -
KFX87_RS13010 (KFX87_013000) 2672167..2673171 + 1005 WP_064145446.1 fimbrial protein -
KFX87_RS13015 (KFX87_013005) 2673313..2674605 + 1293 WP_064145447.1 glycoside hydrolase family 10 protein -
KFX87_RS13020 (KFX87_013010) 2674622..2675944 - 1323 WP_002904792.1 type II toxin-antitoxin system HipA family toxin Toxin
KFX87_RS13025 (KFX87_013015) 2675944..2676210 - 267 WP_002904790.1 type II toxin-antitoxin system antitoxin HipB Antitoxin
KFX87_RS13030 (KFX87_013020) 2676598..2676924 - 327 WP_002904788.1 hydroxyisourate hydrolase -
KFX87_RS13035 (KFX87_013025) 2676921..2677421 - 501 WP_064145448.1 2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase -
KFX87_RS13040 (KFX87_013030) 2677399..2678778 - 1380 WP_064145449.1 nucleobase:cation symporter-2 family protein -
KFX87_RS13045 (KFX87_013035) 2679124..2680278 + 1155 WP_023282572.1 FAD-dependent urate hydroxylase HpxO -
KFX87_RS13050 (KFX87_013040) 2680259..2681182 - 924 WP_064145450.1 LysR family hpxDE operon transcriptional regulator HpxR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Integrative and Conjugative Element - mgtC / iroE 2500223..2711168 210945


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HipA-like, C-terminal

(149-402)

Antitoxin

Cro/C1-type helix-turn-helix domain

(16-65)


Sequences

Toxin        


Download         Length: 441 a.a.        Molecular weight: 49171.46 Da        Isoelectric Point: 7.5357

>T224329 WP_002904792.1 NZ_CP086664:c2675944-2674622 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Download         Length: 1323 bp

>T224329 NZ_CP119986:c909278-909021 [Salmonella enterica]
ATGAATGCCTCCGGCGTCAGCGTCCGCCACATCAACAGCGAAACCTGCATGACCACCAGTTACTCACATATCCCCAGCCA
GCACCTTAAGGGTGACTGGCAGGAAGAAGCGGGATTTGAGACCGGGCGCAGCGTGACCGTGAAGATTTCACAGGGGTGTA
TTGTGCTGATGGCGGACAGTAACGAGGAGCAGAAGCTGCGCGAACAGCTTTACAAGGCTGAACAGGTGGTTAAGGGGATG
CGGGACGTGATTGTTTAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9906.27 Da        Isoelectric Point: 8.5912

>AT224329 WP_002904790.1 NZ_CP086664:c2676210-2675944 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Download         Length: 267 bp

>AT224329 NZ_CP119986:909103-909435 [Salmonella enterica]
TCCGCCATCAGCACAATACACCCCTGTGAAATCTTCACGGTCACGCTGCGCCCGGTCTCAAATCCCGCTTCTTCCTGCCA
GTCACCCTTAAGGTGCTGGCTGGGGATATGTGAGTAACTGGTGGTCATGCAGGTTTCGCTGTTGATGTGGCGGACGCTGA
CGCCGGAGGCATTCATATTTGCTGACTAAATAAATTCTTAATTCTCCGCCGGATGCTGGCTGATTGTGGAGCTCAGGGTG
AGCGAGTATGGGCGCGACATCCTGGCACCGCGCCTCCCTCTCCTCGGCCAGCACCGGGCGGTGATGAGCAACCGGCTACC
GGGGCCGTATTTT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E1CEC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GXD8

References