Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4331416..4332034 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LOE88_RS20770 Protein ID WP_001291435.1
Coordinates 4331416..4331634 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LOE88_RS20775 Protein ID WP_000344800.1
Coordinates 4331660..4332034 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS20735 (4326706) 4326706..4327278 + 573 WP_000779826.1 YbaY family lipoprotein -
LOE88_RS20740 (4327309) 4327309..4327620 - 312 WP_000409911.1 MGMT family protein -
LOE88_RS20750 (4327999) 4327999..4328352 + 354 WP_000878140.1 DUF1428 domain-containing protein -
LOE88_RS20755 (4328394) 4328394..4329944 - 1551 WP_024166621.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LOE88_RS20760 (4330108) 4330108..4330578 - 471 WP_000136192.1 YlaC family protein -
LOE88_RS20765 (4330694) 4330694..4331245 - 552 WP_000102556.1 maltose O-acetyltransferase -
LOE88_RS20770 (4331416) 4331416..4331634 - 219 WP_001291435.1 HHA domain-containing protein Toxin
LOE88_RS20775 (4331660) 4331660..4332034 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LOE88_RS20780 (4332580) 4332580..4335729 - 3150 WP_001132469.1 efflux RND transporter permease AcrB -
LOE88_RS20785 (4335752) 4335752..4336945 - 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224315 WP_001291435.1 NZ_CP086662:c4331634-4331416 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224315 NZ_CP119984:c2487278-2487175 [Salmonella enterica]
GGCAAGGCGATTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224315 WP_000344800.1 NZ_CP086662:c4332034-4331660 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224315 NZ_CP119984:2487173-2487316 [Salmonella enterica]
ATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGC
ATTAATGCAGGCTAAATCGCCTTGCCCTTTAAGAATAGATGACGACGCCAGGTTTTCCAGTTTG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References