Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 2132977..2133631 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name cptA Uniprot ID F4NJ21
Locus tag LOE88_RS10155 Protein ID WP_000244772.1
Coordinates 2132977..2133384 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag LOE88_RS10160 Protein ID WP_000354046.1
Coordinates 2133365..2133631 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS10135 (2128934) 2128934..2130667 - 1734 WP_023155905.1 single-stranded-DNA-specific exonuclease RecJ -
LOE88_RS10140 (2130673) 2130673..2131383 - 711 WP_000715214.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LOE88_RS10145 (2131408) 2131408..2132304 - 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LOE88_RS10150 (2132416) 2132416..2132937 + 522 WP_001055874.1 flavodoxin FldB -
LOE88_RS10155 (2132977) 2132977..2133384 - 408 WP_000244772.1 protein YgfX Toxin
LOE88_RS10160 (2133365) 2133365..2133631 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
LOE88_RS10165 (2133874) 2133874..2134854 + 981 WP_000886062.1 tRNA-modifying protein YgfZ -
LOE88_RS10170 (2134931) 2134931..2135590 - 660 WP_000250274.1 hemolysin III family protein -
LOE88_RS10175 (2135754) 2135754..2136065 - 312 WP_001182954.1 N(4)-acetylcytidine aminohydrolase -
LOE88_RS10180 (2136110) 2136110..2137543 + 1434 WP_001363803.1 6-phospho-beta-glucosidase BglA -
LOE88_RS10185 (2137600) 2137600..2138343 - 744 WP_023155906.1 SDR family oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16048.02 Da        Isoelectric Point: 11.2511

>T224307 WP_000244772.1 NZ_CP086662:c2133384-2132977 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWCIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224307 NZ_CP119983:c49046-48630 [Salmonella enterica]
GTGAAGAAAATGTATATGCTCGACACGAACATCTGCTCGTTCATCATGCGCGAGCAGCCCGAAGCGGTGCTGAAGCGCCT
GGAGCAGGCGGTGCTGCGCGGCCACCGTATTGTGGTCTCGGCCATCACCTACTCAGAGATGCGCTTCGGCGCCACCGGCC
CGAAGGCCTCGCCGCGTCACATTGAACTGGTTGACGCGTTCTGCGCCCGCCTCGATGCTATCCTGCCCTGGGACCGGGCC
GCGGTGAACGCCACCACGGAGATTAAGGTGGCGCTACGCCTCGCCGGGACGCCGATCGGCCCGAACGACACGGCGATCGC
CGGGCACGCTATTGCCGCCGGCGCGGTACTGGTGACAAATAATACGCGGGAGTTTGAGCGGGTGCCGGGTCTTGTTCTGG
AAGACTGGGGAAGATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT224307 WP_000354046.1 NZ_CP086662:c2133631-2133365 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224307 NZ_CP119983:c49273-49043 [Salmonella enterica]
ATGAGAACCGTATCTATTTTTAAAAACGGCAACAACCGCGCCATCCGCCTTCCCCGCGATCTGGATTTTGATGGAGTGAG
CGAGCTGGAAATCGTCCGGGAAGGGGACAGCATCATCCTGCGCCCTGTCCGGCCGACCTGGGGCTCGTTCGCGCAGCTCG
AAAAAGCTGACCCGGGCTTTATGGCGGAGCGTGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XYB4

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References