Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VagC
Location 1657308..1657933 Replicon chromosome
Accession NZ_CP086662
Organism Escherichia coli strain E2

Toxin (Protein)


Gene name vapC Uniprot ID U9YZ02
Locus tag LOE88_RS07960 Protein ID WP_000911329.1
Coordinates 1657308..1657706 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID U9XQV3
Locus tag LOE88_RS07965 Protein ID WP_000450524.1
Coordinates 1657706..1657933 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LOE88_RS07940 (1653186) 1653186..1653386 + 201 WP_000383836.1 YpfN family protein -
LOE88_RS07945 (1653496) 1653496..1654194 - 699 WP_000679823.1 esterase -
LOE88_RS07950 (1654268) 1654268..1656283 - 2016 WP_000829294.1 tRNA cytosine(34) acetyltransferase TmcA -
LOE88_RS07955 (1656298) 1656298..1657161 - 864 WP_001267498.1 neutral zinc metallopeptidase -
LOE88_RS07960 (1657308) 1657308..1657706 - 399 WP_000911329.1 type II toxin-antitoxin system VapC family toxin Toxin
LOE88_RS07965 (1657706) 1657706..1657933 - 228 WP_000450524.1 toxin-antitoxin system antitoxin VapB Antitoxin
LOE88_RS07970 (1658087) 1658087..1658800 - 714 WP_001295467.1 phosphoribosylaminoimidazolesuccinocarboxamide synthase -
LOE88_RS07975 (1659013) 1659013..1660047 - 1035 WP_001297320.1 outer membrane protein assembly factor BamC -
LOE88_RS07980 (1660064) 1660064..1660942 - 879 WP_001295469.1 4-hydroxy-tetrahydrodipicolinate synthase -
LOE88_RS07985 (1661088) 1661088..1661660 + 573 WP_000176187.1 glycine cleavage system transcriptional repressor -
LOE88_RS07990 (1661660) 1661660..1662130 + 471 WP_001068682.1 thioredoxin-dependent thiol peroxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(5-125)

Antitoxin

SpoVT-AbrB domain

(7-52)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14924.23 Da        Isoelectric Point: 8.5325

>T224305 WP_000911329.1 NZ_CP086662:c1657706-1657308 [Escherichia coli]
MLKFMLDTNICIFTIKNKPVHVRERFNLNSGRMCISSVTLMELIYGAEKSQMPERNLAVIEGFVSRLEVLDYDSAAATHT
GQIRAELARQGRPVGPFDQMIAGHARSRGLIVVTNNTREFERVAGIRLEDWS

Download         Length: 399 bp

>T224305 NZ_CP119983:14789-15121 [Salmonella enterica]
ATGGACAGAGGGGAAATCTGGCTCGTCTCACTGGATCCTACAGCCGGACATGAACAAAGCGGAAAACGTCCGGTACTTAT
CGTTTCTCCGGCATCATTTAATAAGTTCACCCGGTTACCCGTTGTCGTTCCCGTCACCAGCGGCGGAGACTTTGCCCGCA
CAGCCGGTTTTACCGTCTCCCTGGACGGCGCGGGAACAAAAACAACGGGTGTTATTCGCTGCGACCAGCCCAGAACGATT
GATATGGGTGCCCGGAATGGTAAGCGTCTGGAACGTATACCCGAAGCTGTCGTAAATGAAGTGCTGGCCCGACTGGAAGC
CATTCTGAGCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8498.61 Da        Isoelectric Point: 4.3867

>AT224305 WP_000450524.1 NZ_CP086662:c1657933-1657706 [Escherichia coli]
METTVFLSNRSQAVRLPKAVALPEDVKKVEIIAIGRTRIITPAGESWDSWFDGENVSADFMDIRDQPAMQERESF

Download         Length: 228 bp

>AT224305 NZ_CP119983:14531-14788 [Salmonella enterica]
ATGTATACCACTCGTCTGAAAAAGGTTGGCGGATCCGTCATGCTGGCGGTTCCTCCCGCCGTGTTGAAAACGCTGGCTCT
GTCGACAGACAGCGAAGTGGGAATGACCATTGATAATGGCTGCCTGATTATTGAACCCCAGAAACGGCCCCGTTATTCGC
TTGAGGAACTGCTGGCACAGTGCGATCCGCACGCCGAAATGAGCGATGAGGATAGGGAATGGATGGATGCGCCTGCAGTG
GGTAAGGAGATCCTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0XW84

Antitoxin

Source ID Structure
AlphaFold DB A0A829CM33

References