Detailed information of TA system TA022430

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 2121822..2122636
• Replicon: chromosome
• Accession: NC_009800
• Organism: Escherichia coli HS

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A7I6H0K3
• Locus tag: ECHS_RS10630
• Protein ID: WP_000854760.1
• Coordinates: 2122259..2122636 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: ECHS_RS10625
• Protein ID: WP_000005490.1
• Coordinates: 2121822..2122169 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
ECHS_RS10610 (EcHS_A2137)	2117919..2118836	+	918	WP_000250228.1	hypothetical protein	-
ECHS_RS10615 (EcHS_A2138)	2118921..2119793	+	873	WP_001069714.1	GTPase family protein	-
ECHS_RS10620 (EcHS_A2139)	2120165..2121865	+	1701	Protein_2019	AIDA repeat-containing protein	-
ECHS_RS10625	2121822..2122169	+	348	WP_000005490.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
ECHS_RS10630 (EcHS_A2140)	2122259..2122636	+	378	WP_000854760.1	TA system toxin CbtA family protein	Toxin
ECHS_RS10635 (EcHS_A2141)	2122633..2122836	+	204	WP_000761705.1	hypothetical protein	-
ECHS_RS25525 (EcHS_A2142)	2122849..2122962	+	114	WP_032140621.1	DUF957 domain-containing protein	-
ECHS_RS25860	2123778..2124149	+	372	WP_001295631.1	IS110 family transposase	-
ECHS_RS10655	2124603..2124785	+	183	WP_024174802.1	ethanolamine utilization protein	-
ECHS_RS10660 (EcHS_A2145)	2124886..2125215	-	330	WP_000450409.1	DUF496 family protein	-
ECHS_RS10665 (EcHS_A2146)	2125387..2126445	-	1059	WP_001200895.1	FUSC family protein	-
ECHS_RS10670 (EcHS_A2147)	2126643..2127116	-	474	WP_001105407.1	DNA gyrase inhibitor SbmC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (15-110)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14165.19 Da
• Isoelectric Point: 7.3249

>T22430 WP_000854760.1 NC_009800:2122259-2122636 [Escherichia coli HS]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYGLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQSGF
SACTHSQLINSIDILRARRATGLMTRDNYRTVNNITLGKHPEAKR

Nucleotide

• Length: 378 bp

>T22430 NC_009800:2122259-2122636 [Escherichia coli HS]
ATGAAAACATTACCCGACACGCATGTACGGGAGGTATCGTGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGTGGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGTCGGGATTC
AGCGCCTGTACCCACTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCCGGGCAACAGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12816.73 Da
• Isoelectric Point: 8.1256

>AT22430 WP_000005490.1 NC_009800:2121822-2122169 [Escherichia coli HS]
MSETVTSGWQPPDRVPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSGELNPRHQ
HTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 348 bp

>AT22430 NC_009800:2121822-2122169 [Escherichia coli HS]
ATGTCGGAAACAGTAACCTCGGGGTGGCAACCTCCGGACAGGGTACCCTGCACAGTGACGCCCTGTTTCGGCGCCCGTCT
GGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCGGTTCAGAGATGCGGATGCGTACC
CTCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGAAATTAATGCTCACCAGTGGTGAACTGAATCCCCGCCATCAG
CATACCGTGACACTGTATGCGAAGGGGCTGACCTGTGAAGCCGACACCCTCGGCTCCTGTGGTTACGTTTATCTGGCTGT
TTATCCGACACCTGAAACGAAAAAGTAA

Structures

Toxin

• AlphaFold DB: A0A7I6H0K3