Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 4702027..4702524 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag LN356_RS22850 Protein ID WP_001173343.1
Coordinates 4702027..4702200 (-) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag LN356_RS22855 Protein ID WP_000398619.1
Coordinates 4702228..4702524 (-) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN356_RS22840 (4698697) 4698697..4700154 + 1458 WP_001550473.1 dipeptide/tripeptide permease DtpC -
LN356_RS22845 (4700391) 4700391..4701908 + 1518 WP_001550472.1 lysine--tRNA ligase -
LN356_RS22850 (4702027) 4702027..4702200 - 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
LN356_RS22855 (4702228) 4702228..4702524 - 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
LN356_RS22860 (4702753) 4702753..4703025 - 273 WP_000405647.1 GNAT family N-acetyltransferase -
LN356_RS22865 (4703037) 4703037..4703267 - 231 WP_001550470.1 4Fe-4S mono-cluster protein YjdI -
LN356_RS22870 (4703447) 4703447..4705078 + 1632 WP_001216477.1 sensor histidine kinase -
LN356_RS22875 (4705075) 4705075..4705794 + 720 WP_000611288.1 two-component system response regulator DcuR -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T224276 WP_001173343.1 NZ_CP086627:c4702200-4702027 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T224276 NZ_CP119967:5618032-5618310 [Pseudomonas sp. A34-9]
ATGGTCAAATCCTTTCAGCACAAAGGCCTTCGCGGCTTCTATGAAACAGGTTCGACTCGCGGGATTCGTGCCGACCACGC
AAAACGGCTGTCGCGCATGCTGCAGTTCATGGATCGCGCTACGATTCCCGCAGACTTGGATCTTCCGGGCTGGCGCCTGC
ATCCATTGAACGGTGAGCTGACGGAGTACTGGTCGCTGAGCGTTTCAGGCAACTGGCGAGTGATCTTTCGTTTTGTCGGT
TCAGACATCGAATTGGTCGATTATCTGGACTACCTCTGA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT224276 WP_000398619.1 NZ_CP086627:c4702524-4702228 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT224276 NZ_CP119967:5618325-5618612 [Pseudomonas sp. A34-9]
ATGCCCATGCACAACCCGCCACACCCCGGTGAAACACTGCTGATGGATGTCTTGCCCGAGCTTGGCATCAGCGTGACCGA
ATTAGCCCGACACTTGGGCTTCGCACGCCCGCATCTTTCACGTGTATTACACGGACATGCGCCAATCAGCCCCGATCTGG
CTGTGCGTCTTGAGCGTGCCGGTATTGGCAAGGCGCGCGTGTGGCTAGGCGTACAAACCGACTATGATCTGTGGCAAGCG
GAGCATCGGGAGCAACCGGCGATTGAACCTATCGCCGCGCACGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References