Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 4450851..4451427 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name shpA Uniprot ID D3GXZ2
Locus tag LN356_RS21565 Protein ID WP_001297643.1
Coordinates 4451140..4451427 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A080I7H7
Locus tag LN356_RS21560 Protein ID WP_000063160.1
Coordinates 4450851..4451153 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN356_RS21545 (4447447) 4447447..4449597 + 2151 WP_001340937.1 pyruvate/proton symporter BtsT -
LN356_RS21550 (4449647) 4449647..4449850 + 204 WP_000467859.1 YbdD/YjiX family protein -
LN356_RS21555 (4449861) 4449861..4450817 + 957 WP_001297640.1 GTPase -
LN356_RS21560 (4450851) 4450851..4451153 - 303 WP_000063160.1 BrnA antitoxin family protein Antitoxin
LN356_RS21565 (4451140) 4451140..4451427 - 288 WP_001297643.1 BrnT family toxin Toxin
LN356_RS25160 (4451626) 4451626..4452322 - 697 Protein_4228 IS1 family transposase -
LN356_RS21580 (4452637) 4452637..4453869 + 1233 WP_001137031.1 multidrug efflux MFS transporter MdtM -
LN356_RS21585 (4453910) 4453910..4455190 + 1281 WP_001037390.1 DUF445 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 4451626..4451835 209


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(37-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11295.77 Da        Isoelectric Point: 6.8821

>T224274 WP_001297643.1 NZ_CP086627:c4451427-4451140 [Escherichia coli]
MPMEFEWDENKAKSNRVKHGIRFEDAVLLFDDPQHLSQQERIENGEYRWQTIGLVYGIVVILVAHTIRFESGNEIIRIIS
ARKADRKERNRYEHG

Download         Length: 288 bp

>T224274 NZ_CP119967:c2482335-2482066 [Pseudomonas sp. A34-9]
ATGCAGGTTGAGTGGCAGCCCGAAGCTCGGGCTGAACTCCTGAAAATCCTTGATTACATCAGTGACCGGAACTTAGTAGC
GGCTGACAGACTTTCCCAAGCTATCGAAGGCGCTACGGTTACCCTTGCTCAACATCCACACCTCTATCGAATAGGTCGAG
CGCCCGGTACTCGCGAGATGGTTGTGCACCCCAATTACCTGATCATTTATCAAGTGGTGGGTCGGATAAATATTCTGAGC
GTTTTGCATGCGCGGCAGGAATATCCATAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11552.01 Da        Isoelectric Point: 9.4646

>AT224274 WP_000063160.1 NZ_CP086627:c4451153-4450851 [Escherichia coli]
MSMVKHKRGTSSTLDVQHEAELKALTNKSDEDIDYSDIPPSSDEQWSNAERGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMLRDQNKK

Download         Length: 303 bp

>AT224274 NZ_CP119967:c2482531-2482325 [Pseudomonas sp. A34-9]
ATGATCGACCCGTCTTTAATCGACCCACTGTGCCCAGTTGTTTCAGATCTTGATCCTGAAGAGCAGGCCGCGAGCTATGA
CCGTTGGTTAATCGCTAAAGTGCAGGCTTCGATGAGTGATACAAGACCGAGCATTCCCAATGATCAGGTTATGGCCGAGA
TGCGTGCCTTGATGGAAGCAAAGCGTAAAAAATACGATGCAGGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829GEV9

Antitoxin

Source ID Structure
AlphaFold DB A0A080I7H7

References