Detailed information of TA system TA224272

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 4097536..4099225
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: V0Z9M0
• Locus tag: LN356_RS19900
• Protein ID: WP_000365809.1
• Coordinates: 4097536..4098456 (-)
• Length: 307 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: LN356_RS19905
• Protein ID: WP_001550661.1
• Coordinates: 4098443..4099225 (-)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS19885 (4093626)	4093626..4094753	-	1128	WP_001550663.1	MFS transporter	-
LN356_RS19890 (4094895)	4094895..4095953	-	1059	WP_122987197.1	alpha/beta hydrolase	-
LN356_RS19895 (4096218)	4096218..4097102	+	885	WP_001119037.1	LysR family transcriptional regulator	-
LN356_RS19900 (4097536)	4097536..4098456	-	921	WP_000365809.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
LN356_RS19905 (4098443)	4098443..4099225	-	783	WP_001550661.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
LN356_RS19910 (4099541)	4099541..4102750	+	3210	WP_001521910.1	RNA-directed DNA polymerase	-
LN356_RS19915 (4103097)	4103097..4103567	+	471	WP_000201185.1	hypothetical protein	-
LN356_RS19920 (4103564)	4103564..4103666	+	103	Protein_3909	helix-turn-helix domain-containing protein	-
LN356_RS19925 (4103758)	4103758..4103916	+	159	Protein_3910	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-261)

Antitoxin

• Transcriptional regulator, AbiEi 3 antitoxin, Type IV TA system (95-247)

Sequences

Toxin

Protein

• Length: 307 a.a.
• Molecular weight: 34571.01 Da
• Isoelectric Point: 7.2649

>T224272 WP_000365809.1 NZ_CP086627:c4098456-4097536 [Escherichia coli]
MDLRDSYARQVKLLMAALPYVAKESCFALKGGTAINLFVQDFPRLSVDIDLAYKSFADRDTDLAAINDALKRITASLNGR
PGITAIRQENKADEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQH
PRDLFDVLNMLEKPGLMREIFDGFLCYLAGHPRPIAELLAPNWDTERIATLYAQEFSGMTQQETSLESLLSVTTLLPQAL
KSHLTDLDREFLLSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 921 bp

>T224272 NZ_CP119967:212371-212652 [Pseudomonas sp. A34-9]
ATGACCTATAAGCTTGAATTTCTGCCGTCTGCTCACAAAGAGTGGAACAAGTTGGGTCATACACTACGAGAACAATTCAA
AAAGAAGCTGGGCGAGAGGTTGAAGCTGCCAAGGGTTTCTGCTGATGCTCTGCACGGGATGCCGGACTGCTACAAAATCA
AACTCAAAGCTTCCGGCTATCGCTTGGTATATCAGGTGATCGATGAGCGCGTTGTTGTTTCGGTTGTCGCAGTTGGTAAG
CGCGAGCGCAGCAGCGTTTACGAGAATGCAAAAAAGCGCTGA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29191.43 Da
• Isoelectric Point: 8.9059

>AT224272 WP_001550661.1 NZ_CP086627:c4099225-4098443 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQSQWEKPVRVAGLT
SLALQGHAHYTEQGKPQVWLALPAYVKLPGWFSAFEQSATFVTLYTSGLTADVTPFTTELKIGDRQLAGSVPELAAYEIA
CGVPSLISFEHADALFQGLNLLSPRKLQALLRASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT224272 NZ_CP119967:212136-212381 [Pseudomonas sp. A34-9]
ATGCAAAGCGTTCTGGCCGATATGGCTGTCAGTGTCTCAGAATTAAAGAAAAACCCATCCGCAGTGCTGAGCGGCGCACA
TGGCGGGGCTGTGGCCGTTCTTAACCACAATCGCGTCATGGGCTACATGGTGCCAGCAGACGTTTACGAAGCAATGATGG
AGCGGCTTGATGATCTGGAGCTTGCTGAAATCGTTCGCGCCCGCAGCCATGAGACGCCAATTTCGGTAAGCCTGGATGAC
CTATAA

Structures

Toxin

• AlphaFold DB: V0Z9M0