Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3892867..3893485 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag LN356_RS18940 Protein ID WP_001291435.1
Coordinates 3893267..3893485 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag LN356_RS18935 Protein ID WP_000344800.1
Coordinates 3892867..3893241 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN356_RS18925 (3887956) 3887956..3889149 + 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
LN356_RS18930 (3889172) 3889172..3892321 + 3150 WP_001132480.1 efflux RND transporter permease AcrB -
LN356_RS18935 (3892867) 3892867..3893241 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
LN356_RS18940 (3893267) 3893267..3893485 + 219 WP_001291435.1 HHA domain-containing protein Toxin
LN356_RS18945 (3893657) 3893657..3894208 + 552 WP_000102564.1 maltose O-acetyltransferase -
LN356_RS18950 (3894324) 3894324..3894794 + 471 WP_000136192.1 YlaC family protein -
LN356_RS18955 (3894958) 3894958..3896508 + 1551 WP_001366446.1 cyclic-guanylate-specific phosphodiesterase PdeB -
LN356_RS18960 (3896550) 3896550..3896903 - 354 WP_085334692.1 DUF1428 family protein -
LN356_RS18970 (3897282) 3897282..3897593 + 312 WP_000409911.1 MGMT family protein -
LN356_RS18975 (3897624) 3897624..3898196 - 573 WP_000779831.1 YbaY family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T224271 WP_001291435.1 NZ_CP086627:3893267-3893485 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T224271 NZ_CP119871:18520-19404 [Methylosinus trichosporium OB3b]
GTGCGAGACCGGCTGACCCAGCGTGCGCGGGCCGCCGGCGAGAATGTTCAGCTGATCCTGACCCGCTACGCGATCGAAAG
GCTCCTTTACCGGCTGAGCGCTTCACAACATCGAGAACGGTTCGTCCTGAAGGGCGCCATGCTCTTCAGCCTGTGGGGGC
CGACGCCTTATCGCGCGACTGGAGACCTCGATCTTTTCGGGACAGGCAACCCCGAGATCGAACAATTGATCTCCGTCTTT
CGAGCAATCATTCAGGTCGAAGTCGTCGAAGACGGCGTCGCCTTCCGATCCGACACTTTGCGCGCCGAGCCGACGCGACC
ACAAGACGAATATGCAGGCGTTCGGATCATGTTCGACGCCTCGATCGCCAATGCTCGGCTCCCGATCCAAGTGGACATCG
GATTCGGCGACGCCATCACCCCTGCCCCGCAGGAGATCGAATATCCATCGCTGATCGGAATGCCGGCGCCGAAACTCAAG
GCCTACCCGCCAGAGACAGTCGTCGCGGAAAAGCTCGAGGCTCTCGTATCGCTCGGCGTGGCCAACAGCAGGATGAAGGA
TTTCTACGATCTCTGGGCGATCAGTCGGACCTTCGCCTTCGATGGGCTGGTGCTGACCAAAGCGATACGGGCGACATTCG
AGCGCCGCGGAACGATGCTGCCGACCGAAATTCCATTCGGACTTTCAGAGGCGTTCGCTGCGGATCCGGTCAAGCAAACA
CAGTGGCGGGCCTTCCTTCGGCGCACCGAGATCGCGATGGCGCCGGAGCCCCTGACCCAAATTATCCCGTCGATCGCCTC
ATTTCTGATGCCGGTCCTTCAGTCCGCCGCCGACGAACGGACCACTCCAGGAAAATGGCCGGTCGGCGGGCCTTGGGGAG
ATTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT224271 WP_000344800.1 NZ_CP086627:3892867-3893241 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT224271 NZ_CP119871:17864-18487 [Methylosinus trichosporium OB3b]
ATGCAACAATCGCCCACGATCCAGGCCAAAAGCCTGAAGGAGCTCGCCCTCGCCGTCGCTCGCGACCGCGGGATAGCTCG
TTCGCGGGATTTCGAAGCGGCAGGAGTTCCGCGCATCTACCTTCAACGCCTCCGGGACGAGGGACTACTCACGCAACCCG
GAAGGGGCCTCTACGCGCTCGCCAACAGCCAAATCAGCGCGCATCACAGTCTCGCCGAGGCTGCCAAATCTGTTCCCAGC
GGGGTCATTGGACTGCTTTCGGCTCTCCAGTTCCATGAACTGACGACCCAGCTCCCGTCCCAGGTCTGGATGCTCCTGCC
TTCAAAGGCATGGGCGCCGAGGCGATCGCCCGTCACACTGAAGATTCTGCGCGCCAGTGGTGAGGCGCTGAAAGCCGGAG
TCGAGCAGCAGCTCGTCGATCGGGTGGTCGTCCCCATCACATCGCCTGCGAAAACCGTGGCCGACTGTTTCAAATATCGC
GGCAAAATCGGTCTCGACGTGGCTATCGAGGCGCTTCGGGACTGCCTGACGAAAAAGCGGGCCACGCGCGACGAAATCTG
GCGTTACGCCGCCATCGATCGCGTCCAGAATGTCATGCGGCCTTATCTGGAGGCGCTCTCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References