Detailed information of TA system TA224270

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 3439869..3440574
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: LN356_RS16825
• Protein ID: WP_000539521.1
• Coordinates: 3439869..3440255 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: LN356_RS16830
• Protein ID: WP_001280945.1
• Coordinates: 3440245..3440574 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS16805 (3435873)	3435873..3436499	+	627	WP_001295292.1	glutathione S-transferase GstB	-
LN356_RS16810 (3436496)	3436496..3437611	-	1116	WP_000555051.1	aldose sugar dehydrogenase YliI	-
LN356_RS16815 (3437722)	3437722..3438105	-	384	WP_000497137.1	biofilm formation regulator BssR	-
LN356_RS16820 (3438318)	3438318..3439643	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
LN356_RS16825 (3439869)	3439869..3440255	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
LN356_RS16830 (3440245)	3440245..3440574	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
LN356_RS16835 (3440644)	3440644..3441972	-	1329	WP_001550887.1	GGDEF domain-containing protein	-
LN356_RS16840 (3441980)	3441980..3444328	-	2349	WP_001550886.1	EAL domain-containing protein	-
LN356_RS16845 (3444506)	3444506..3445417	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T224270 WP_000539521.1 NZ_CP086627:3439869-3440255 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T224270 NZ_CP119870:c23781-23380 [Methylosinus trichosporium OB3b]
TTGACCCGCTATCTGCTGGATACGAACATCGTCTCCGATTTGATCAGGAATCCGCGAGGCCGGGTCGCCGCACATATCGC
TCATGTGGGCGAGGCCAATGTCTGCACCAGCGTCATCGTGGCCGCCGAGCTGCGCTACGGCTGCGCCAAGAGCGGCTCGA
AGCGGCTCTCCGCGGCGGTCGAGAGCCTGCTGGGAGAGCTGGCCGTGCTTGCTCTGGAAGGCCCCGCCGCCGCCGAATAT
GGGGCGATCCGGGCCGGGCTGGAACGACGGGGAACCCCGATCGGCGGCAATGACCTATTGATCGCCGCTCATGCTCTGGC
GATCGATGCGACGGTCGTGACCGCCAATATGGACGAATTCGCCCGAGTCGAGGGGCTGAAGGTGCAGAACTGGCTCGCAT
AG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT224270 WP_001280945.1 NZ_CP086627:3440245-3440574 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT224270 NZ_CP119870:c24014-23778 [Methylosinus trichosporium OB3b]
ATGGGCGAGGCGCGGCATGTGAAGCTGTTTCGGAACGGGCGCAACCAGGCGGTGCGCATTCCCGTCGAGTTCGAGCTGCC
CGGCGACGAGGCGATCATGCGCCGCGAGGGCGATCGTCTCGTGCTGGAGCCGACGCGCAAGCGCGGTCTCCTGGGCCTGC
TCGAGACGATGGAGCCCATCGACGAGGCCTTCCCCGAGATCGAAGATCAGCCGCCCGCGCCGGAGCCGATCTTTTGA

Structures

Toxin

• AlphaFold DB: A0A829GCT6