Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) tacAT/DUF1778(antitoxin)
Location 3177575..3178359 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name TacT2 Uniprot ID V0T0H9
Locus tag LN356_RS15570 Protein ID WP_000613626.1
Coordinates 3177865..3178359 (+) Length 165 a.a.

Antitoxin (Protein)


Gene name TacA2 Uniprot ID L4JCW6
Locus tag LN356_RS15565 Protein ID WP_001110447.1
Coordinates 3177575..3177868 (+) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN356_RS15555 (3172725) 3172725..3173684 - 960 WP_000846342.1 23S rRNA pseudouridine(955/2504/2580) synthase RluC -
LN356_RS15560 (3174257) 3174257..3177442 + 3186 WP_001550951.1 ribonuclease E -
LN356_RS15565 (3177575) 3177575..3177868 + 294 WP_001110447.1 DUF1778 domain-containing protein Antitoxin
LN356_RS15570 (3177865) 3177865..3178359 + 495 WP_000613626.1 GNAT family N-acetyltransferase Toxin
LN356_RS15575 (3178454) 3178454..3179407 - 954 WP_001212768.1 flagellar hook-associated protein FlgL -
LN356_RS15580 (3179419) 3179419..3181062 - 1644 WP_000096478.1 flagellar hook-associated protein FlgK -
LN356_RS15585 (3181128) 3181128..3182069 - 942 WP_001366226.1 flagellar assembly peptidoglycan hydrolase FlgJ -
LN356_RS15590 (3182069) 3182069..3183124 - 1056 WP_229321855.1 flagellar basal body P-ring protein FlgI -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(81-144)

Antitoxin

Vibrio phage ICP1, Orf50

(14-88)


Sequences

Toxin        


Download         Length: 165 a.a.        Molecular weight: 18084.00 Da        Isoelectric Point: 8.2617

>T224269 WP_000613626.1 NZ_CP086627:3177865-3178359 [Escherichia coli]
MIQAPEPLMARHQFTSFCSGVETMDNWLKQRALKNQLAGASRTFVSCDTYSNVLAYYSLASSAVETYVATGRFRRNMPEP
IPVVVLGRLAIDKSLQGQGIGRAMVRDAGLRVLQAAEVIGIRGMLVHALSDQAREFYLRVGFEPSPVDSMILMATLADLQ
ECLK

Download         Length: 495 bp

>T224269 NZ_CP119869:4422974-4423252 [Methylosinus trichosporium OB3b]
ATGATACGCTCCGTCAAAGGCACGGTGACTCGGCAGTTCGTCGAGACCGGCAAGTCGAAGTTTTCCGGCCTCGACGAAGC
GCTCGCGCGGCAACGTCTGGCGGAGCTCGACAGCGCCGCCTCGCTCGATGACCTTCCACCGCTTCGTAGCGTCGGACTTC
ACCGGCTCTCGGGTGACCGCAAGGGCCAATGGGCGATCAAAGTGAATGGCCCCTGGCGGATCGCCTTTCGATTCGAGGAC
GGCGATGCGTTCGAGGTGGAGATCGTCGACTATCATTGA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11109.69 Da        Isoelectric Point: 10.5423

>AT224269 WP_001110447.1 NZ_CP086627:3177575-3177868 [Escherichia coli]
MPAANNIPTRRESLNLRIKPAERDLIDRAAKARGKNRTEFVLEAARAAAEEALIEQRIFRIDSDDYQLFLNRLDQPPAPN
AALRKTMQTPAPWEQKK

Download         Length: 294 bp

>AT224269 NZ_CP119869:4423380-4423706 [Methylosinus trichosporium OB3b]
ATGACGACGAAAAGCGCGGACATCTTCGAGGCCCCGCCCGGCGGCTTCCGTTTCGGCCCGATCCATCCGGGCCGCACCCT
CGCCGCCGAGCTGAAAGCGCGCGGAATCAGCGCTCACGCGCTGGCGCTGAGCTTGCGGGTGCCCGCCAACCGGATCAGCG
AGATCGTCGCTGGAAAGCGCGGCGTCACCGCGGAGACCGCGCTGCGCCTCGCCCGCTATCTCGGCACGAGCGCGGCCTTC
TGGATGAATCTGCAGTCGAAATATGATCTCGAGATCGCCGAGCGCGATTTCGGCGAGCGGATCAGGGCGGAAGTCGAAGC
GGCCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0T0H9

Antitoxin

Source ID Structure
AlphaFold DB L4JCW6

References