Detailed information of TA system TA224268

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 2794064..2794702
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: A0A7U9DHD1
• Locus tag: LN356_RS13570
• Protein ID: WP_000813795.1
• Coordinates: 2794526..2794702 (-)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: LN356_RS13565
• Protein ID: WP_076797675.1
• Coordinates: 2794064..2794480 (-)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS13545 (2789216)	2789216..2790157	-	942	WP_001251315.1	ABC transporter permease	-
LN356_RS13550 (2790158)	2790158..2791171	-	1014	WP_001551095.1	ABC transporter ATP-binding protein	-
LN356_RS13555 (2791189)	2791189..2792334	-	1146	WP_001551094.1	ABC transporter substrate-binding protein	-
LN356_RS13560 (2792579)	2792579..2793985	-	1407	WP_001551093.1	PLP-dependent aminotransferase family protein	-
LN356_RS13565 (2794064)	2794064..2794480	-	417	WP_076797675.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
LN356_RS13570 (2794526)	2794526..2794702	-	177	WP_000813795.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
LN356_RS13575 (2794924)	2794924..2795154	+	231	WP_023910283.1	YncJ family protein	-
LN356_RS13580 (2795246)	2795246..2797207	-	1962	WP_001551090.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
LN356_RS13585 (2797280)	2797280..2797816	-	537	WP_001551089.1	DNA-binding transcriptional regulator SutR	-
LN356_RS13590 (2797908)	2797908..2799080	+	1173	WP_001551088.1	BenE family transporter YdcO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-65)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6747.80 Da
• Isoelectric Point: 11.5666

>T224268 WP_000813795.1 NZ_CP086627:c2794702-2794526 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T224268 NZ_CP119869:c3617947-3617630 [Methylosinus trichosporium OB3b]
ATGTGGTCTGTCGAATATCTGCCCGCTGCGGCCGAAGAGGAAGCGGCGCTTCCGATCGAGATGCAGGCGAGACTCGCCCG
GATGCTCGATGTCATCCGCCGTGCGGGGCTGACAAATTTGCCGCGCGACTGGGTGAAGCCGCTCGAGGGCAAATTGTGGG
AACTGCGGATCACCGGGAAGGACGGCGTCGCCCGCGCCATCTATGTCACAGCTGAAGGACGGCGCGTGGTCATCGTTCGA
ATCTTCGTGAAGAAGACGCAAAAAACGCCGCGGCGCGAGCTGGAGCTCGCTCGACGACGGGCGAAGGAGGTCGAATGA

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15220.55 Da
• Isoelectric Point: 4.7386

>AT224268 WP_076797675.1 NZ_CP086627:c2794480-2794064 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPSNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT224268 NZ_CP119869:c3617633-3617343 [Methylosinus trichosporium OB3b]
ATGACCAAGACGATCGACGCGCGAATCATTCATGCGAAGCGGATGGCCGAAGACGAGGCCTATCGCGCCGCCTATGACGC
GCTCGAAGAGGAGTTCGCTCTCGTCAATTCCATGATCGAGGCGCGAGCGCGTTCCAAATTGTCGCAGGCGGAAGTGGCGA
AGCGCATGGGCACGACGGAAAGCGCGGTGTCGAGGCTCGAATCCGGGCGCGGCAAACCATCGACGCGCACATTGGAACGC
TACGCCGAAGCCGTCGGGCATCGGCTGCGGATCACATTCGAGCCCTCGTGA

Structures

Toxin

• AlphaFold DB: A0A7U9DHD1