Detailed information of TA system TA224267

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2689350..2690938
• Replicon: chromosome
• Accession: NZ_CP086627
• Organism: Escherichia coli strain RIVM_C036569

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A829LC11
• Locus tag: LN356_RS13125
• Protein ID: WP_001551128.1
• Coordinates: 2689616..2690938 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: LN356_RS13120
• Protein ID: WP_001296726.1
• Coordinates: 2689350..2689616 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
LN356_RS13120 (2689350)	2689350..2689616	+	267	WP_001296726.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
LN356_RS13125 (2689616)	2689616..2690938	+	1323	WP_001551128.1	type II toxin-antitoxin system serine/threonine protein kinase toxin HipA	Toxin
LN356_RS13130 (2691138)	2691138..2692490	-	1353	WP_001551127.1	SIR2 family protein	-
LN356_RS13135 (2692705)	2692705..2693737	-	1033	Protein_2579	ISNCY family transposase	-
LN356_RS13140 (2694104)	2694104..2695003	-	900	WP_001551125.1	LysR family transcriptional regulator	-
LN356_RS13145 (2695142)	2695142..2695450	+	309	WP_001221046.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2692705..2693523	818

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-401)

Antitoxin

• Cro/C1-type helix-turn-helix domain (14-69)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49270.88 Da
• Isoelectric Point: 8.2472

>T224267 WP_001551128.1 NZ_CP086627:2689616-2690938 [Escherichia coli]
MPKLVTWMNNQRVGELTKLANGAHTFKYAPEWLANRYARPLSLSLPLQRGNITSDAVFNFFDNLLPDSPIVRDRIVKRYH
AKSRQPFDLLSEIGRDSVGAVTLIPEDETVTHPIMAWEKLTEARLEEVLTAYKADIPLGMIREENDFRISVAGAQEKTAL
LRIGNDWCIPKGITPTTHIIKLPIGEIRQPNATLDLSQSVDNEYYCLLLAKELGLNVPDAEIIKAGNVRALAVERFDRRW
NAERTVLLRLPQEDMCQTFGLPSSVKYESDGGPGIARIMAFLMGSSEALKDRYDFMKFQVFQWLIGATDGHAKNFSVFIQ
AGGSYRLTPFYDIISAFPVLGGAGIHISDLKLAMGVNASKGKKTAIDKIYPRHFLATAKVLKFPEVQMHEILSDFARMIP
AALDNVKTSLPTDFPENVVTAVETNVLRLHGRLSREYGIK

Nucleotide

• Length: 1323 bp

>T224267 NZ_CP119869:3149555-3149995 [Methylosinus trichosporium OB3b]
ATCGAAGCGCTCCTGCGCAACGAACCGCATCGTGATCGCTCGCTGCTGGAGCGCACCCGCGACAGAATGGATCAACATGT
CCGCGACTACCTCGTCACCGGCTATGAACCACTGATCCCATCGCTTGTTCTACCCGACATGAACGATCGCCACGTCCTCG
CGGCGGCGATCGTCGGGCGCTGCGATGTCATAGTGACGCAGAACTTGAAGCACTTTCCTGAAACCGCTCTCGCGCCGCTC
GACATCGATGCGCAGCACCCGGACGAATTCCTGTGCAACCAGCTCGACCTGGCGCCCGGCATCTTTTGCGCGGCCGTCCG
CAAGGTTTGCGCACGGTTGAAAAACCCACTCTATTCCGTTGACGACTACCTCGCCAATCTCACCCGGCAGGGGCTTGTCG
GCGCCGCCTCGGAGCTCGAACAATTTTCTGGGCTCATATAG

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10047.39 Da
• Isoelectric Point: 4.9632

>AT224267 WP_001296726.1 NZ_CP086627:2689350-2689616 [Escherichia coli]
MMSFQKIYSPTQLANAMKLVRQQNGWTQSELAKKIGIKQATISNFENNPDNTTLTTFFKILQSLELSMTLCDTKNASPES
TEQQDLEW

Nucleotide

• Length: 267 bp

>AT224267 NZ_CP119869:3148966-3149430 [Methylosinus trichosporium OB3b]
ATGACCGCGCTCGCTCACCGCCAGTTGCCGCCGACGGCGCAGGACGCCGCCATTGCTCGCACCTCCGGTCAGCGCCTCTC
GCGCTTTGCCCGCAGCAATCGCTCGCTCTCGCTTCGCATCACAGACGCCGAGCAAGAGCAGCCGATCGAGCTGCCGGCCG
GCGCCGTATCGTTGCTCATGGACATTCTAGAGGCTATGGCCGCCGGGCGCGGCGTAACCCTTATTCCTGAAAATGCCGAG
CTGACCAGTGTGCAGGCGGCGGATATTCTGAACGTCTCCCGCCCATTCCTCATCGGGCTTCTCGACTCCAAGGCAATCCC
TCATCACAAGGTCGGCAAGCACCGCCGCATCCGCATGGAGGACGTCATGGCCTATAAGGAGCGGATCGACCGGGAACGGG
AAACGGTCCTCGACCAACTCGTCGCCGACGCTCAGGAACATGACATGGGCTACAGTCGCAAATGA

Structures

Toxin

• AlphaFold DB: A0A829LC11