Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1115078..1115732 Replicon chromosome
Accession NZ_CP086627
Organism Escherichia coli strain RIVM_C036569

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag LN356_RS05525 Protein ID WP_000244781.1
Coordinates 1115325..1115732 (+) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID A0A0V9GG32
Locus tag LN356_RS05520 Protein ID WP_001564007.1
Coordinates 1115078..1115344 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
LN356_RS05500 (1111166) 1111166..1112599 - 1434 WP_001564009.1 6-phospho-beta-glucosidase BglA -
LN356_RS05505 (1112644) 1112644..1112955 + 312 WP_001182966.1 N(4)-acetylcytidine aminohydrolase -
LN356_RS05510 (1113119) 1113119..1113778 + 660 WP_000250274.1 hemolysin III family protein -
LN356_RS05515 (1113855) 1113855..1114835 - 981 WP_001564008.1 tRNA-modifying protein YgfZ -
LN356_RS05520 (1115078) 1115078..1115344 + 267 WP_001564007.1 FAD assembly factor SdhE Antitoxin
LN356_RS05525 (1115325) 1115325..1115732 + 408 WP_000244781.1 protein YgfX Toxin
LN356_RS05530 (1115772) 1115772..1116293 - 522 WP_001055874.1 flavodoxin FldB -
LN356_RS05535 (1116405) 1116405..1117301 + 897 WP_000806638.1 site-specific tyrosine recombinase XerD -
LN356_RS05540 (1117326) 1117326..1118036 + 711 WP_001564005.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
LN356_RS05545 (1118042) 1118042..1119775 + 1734 WP_001564004.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T224256 WP_000244781.1 NZ_CP086627:1115325-1115732 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T224256 NZ_CP119862:c4739272-4738898 [Citrobacter sp. S171]
ATGGCTAAAGGATCGGCACTTTTTGACACCAATATTTTAATCGATTTGTTCAGCGGGCGAGGGGAAGCAAAGCAAGCGCT
GGACGCATGGCCGCCGCAGAATGCGATAAGCCTCATCACCTGGATGGAAGTGCTGGTTGGCGCCAGGAAATACAATCAGG
AACATCGCACACGGGTTGCGATGAGCGCATTTAACATCATTGGTGTTTCGCAGGAGATTGCTGAGCGAAGCGTTGTTCTA
CGGCAGGATTATGGACTGAAGCTTCCGGATGCCATTATTCTGGCTACCGCACAGATTCACCGTCTTGAACTGGTCACGCG
CAATACGAAGGATTTTGCGGGAATACCTGGCGTCGTGACGCCCTATGAACTGTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10565.15 Da        Isoelectric Point: 5.1599

>AT224256 WP_001564007.1 NZ_CP086627:1115078-1115344 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWMMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT224256 NZ_CP119862:c4739514-4739272 [Citrobacter sp. S171]
ATAATGACTGGACTGGATATGGGCAGAATTTTACTCGATTTATCTGATGAAGTGATTAAACGGCTGGACGATCTCAAGGT
GCAGCGCAATCTTCCGCGTGCCGAACTGTTACGGGAAGCGGTTGAGCAGTATCTGGAAAGACAGGATCAGGCAGAAACAA
CAATTTCTAACGCACTTGGCCTGTGGCAAGGCTGTGAAGAAGATGGCGTTGAATACCAGCGCAAACTTCGCGAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
AlphaFold DB A0A0V9GG32

References