Detailed information of TA system
Overview
TA module
| Type | IV | Classification (family/domain) | yeeUV (cbtA-cbeA)/CbtA-CbeA |
| Location | 989314..990109 | Replicon | chromosome |
| Accession | NZ_CP086627 | ||
| Organism | Escherichia coli strain RIVM_C036569 | ||
Toxin (Protein)
| Gene name | yeeV | Uniprot ID | A0A4Y9BN12 |
| Locus tag | LN356_RS04880 | Protein ID | WP_023909482.1 |
| Coordinates | 989314..989688 (-) | Length | 125 a.a. |
Antitoxin (Protein)
| Gene name | yeeU | Uniprot ID | A0A4Y9BHM8 |
| Locus tag | LN356_RS04885 | Protein ID | WP_023909483.1 |
| Coordinates | 989735..990109 (-) | Length | 125 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| LN356_RS04850 (985279) | 985279..986544 | - | 1266 | WP_001218869.1 | integrase arm-type DNA-binding domain-containing protein | - |
| LN356_RS04855 (987008) | 987008..987334 | - | 327 | WP_001522265.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| LN356_RS04860 (987331) | 987331..987594 | - | 264 | WP_001143297.1 | type II toxin-antitoxin system ParD family antitoxin | - |
| LN356_RS04865 (987666) | 987666..988532 | - | 867 | WP_023909479.1 | DUF4942 domain-containing protein | - |
| LN356_RS04870 (988617) | 988617..988814 | - | 198 | WP_001522268.1 | DUF957 domain-containing protein | - |
| LN356_RS04875 (988826) | 988826..989317 | - | 492 | WP_023909481.1 | DUF5983 family protein | - |
| LN356_RS04880 (989314) | 989314..989688 | - | 375 | WP_023909482.1 | TA system toxin CbtA family protein | Toxin |
| LN356_RS04885 (989735) | 989735..990109 | - | 375 | WP_023909483.1 | type IV toxin-antitoxin system cytoskeleton bundling-enhancing antitoxin CbeA | Antitoxin |
| LN356_RS04890 (990189) | 990189..990410 | - | 222 | WP_023909484.1 | DUF987 domain-containing protein | - |
| LN356_RS04895 (990462) | 990462..991217 | - | 756 | WP_001522280.1 | IS21-like element ISEc10 family helper ATPase IstB | - |
| LN356_RS04900 (991214) | 991214..992713 | - | 1500 | WP_059332257.1 | IS21-like element ISEc10 family transposase | - |
| LN356_RS04905 (992894) | 992894..993370 | - | 477 | WP_001186724.1 | RadC family protein | - |
| LN356_RS04910 (993385) | 993385..993858 | - | 474 | WP_001522284.1 | antirestriction protein | - |
| LN356_RS04915 (993953) | 993953..994129 | - | 177 | WP_001522285.1 | hypothetical protein | - |
| LN356_RS04920 (994129) | 994129..994947 | - | 819 | WP_001522287.1 | DUF932 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Integrative and Conjugative Element | - | gspC / gspD / gspE / gspF / gspG / gspH / gspI / gspJ / gspK / gspL / gspM / kpsM / kpsT / ugd / kpsS / kpsC / kpsU / kpsD / kpsE / kpsF / papX | 797594..992637 | 195043 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 125 a.a. Molecular weight: 14009.98 Da Isoelectric Point: 8.2905
>T224255 WP_023909482.1 NZ_CP086627:c989688-989314 [Escherichia coli]
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
MKTLSDTHVREASRCPSPVTIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRSNYRTVNNITQGKHPEAK
Download Length: 375 bp
>T224255 NZ_CP119862:3576904-3577122 [Citrobacter sp. S171]
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACTATTGACACGCTTGAACGTGT
TATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGACCACCGTCTCGCAGAAT
TGACGATGAATAAGCTTTACGACAAGATCCCCACCTCGGTCTGGAAATTTATTCGTTAA
ATGTCCGATAAACCATTAACTAAAACTGATTATTTGATGCGCTTGCGACGCTGTCAGACTATTGACACGCTTGAACGTGT
TATTGAGAAAAATAAATATGAATTATCTGACAATGAATTGGCTGTATTTTACTCAGCAGCAGACCACCGTCTCGCAGAAT
TGACGATGAATAAGCTTTACGACAAGATCCCCACCTCGGTCTGGAAATTTATTCGTTAA
Antitoxin
Download Length: 125 a.a. Molecular weight: 13598.31 Da Isoelectric Point: 5.5051
>AT224255 WP_023909483.1 NZ_CP086627:c990109-989735 [Escherichia coli]
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
VSDTLPGTTLPDDNNDRTWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHPDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPAPATTS
Download Length: 375 bp
>AT224255 NZ_CP119862:3576502-3576876 [Citrobacter sp. S171]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACATCGGCGATCAATCTTCAGCTTAATGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATATCTG
GACGATACGTTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTTCAGAAATGGCGAAAGTCGGGAAACCGCCT
GTTTCGCTGTTTTGTGAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGTCTTGC
AAACCTTGAAGAAAGCAACCATGGCTGGGTTAACGACCCAACATCGGCGATCAATCTTCAGCTTAATGAGCTGATAGAGC
ATATTGCAACCTTCGCACTTAATTATAAAATTAAGTACAATGAGGACAATAAGCTGATTGCGCAGATAGATGAATATCTG
GACGATACGTTTATGTTGTTCAGCAGTTATGGCATTAATGCGCAGGATCTTCAGAAATGGCGAAAGTCGGGAAACCGCCT
GTTTCGCTGTTTTGTGAACGCCACCAGGGCTAATCCTGTTAGTTTGTCTTGTTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BN12 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A4Y9BHM8 |